LOCUS       sequence-273534-        4281 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4281)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-026   Lyn-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..4281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1197..1216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1665..1786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1702..1721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1756..1775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2587..2616
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2638..3225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3126..3145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3237..3264)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3288..4079,4080..4148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3911..3930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4149..4251)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4252..4281,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgggctgcat caagagcaag aggaaagacg tggaaaacgt cgagggcggc agcgtgtcaa
      901 agggagaaga gttgtttacc ggtgttgttc ccatactggt ggaactggat ggtgacgtca
      961 atggacacaa gtttagcgtt tctggcgaag gagaaggcga tgctacctat ggcaaactga
     1021 ccctcaaact gatctgtacg actggaaagt tgcctgtacc ttggccaaca ctggtcacaa
     1081 ccttgggcta tgggctgcaa tgctttgccc gttatcctga tcacatgaag caacatgact
     1141 tctttaagtc tgctatgcca gagggatatg tgcaggaacg gactatcttc ttcaaggacg
     1201 acggcaacta caagacgaga gctgaggtga aatttgaggg tgacacactg gtgaatcgca
     1261 tagagctgaa aggcatcgac ttcaaagaag atgggaatat tctcgggcat aaactcgagt
     1321 acaactacaa ttcccacaac gtgtacatca ctgccgataa gcagaagaat gggattaagg
     1381 cgaacttcaa gatccgacac aacatcgagg atggtggggt ccagcttgcc gaccattatc
     1441 agcagaacac acccattggg gatggaccag tcctgttgcc ggataaccac tacctcagtt
     1501 accagagcaa actgtccaaa gatcccaatg agaagaggga ccatatggta ctgctcgaat
     1561 tcgtgactgc agcaggcatt acccttggga tggatgaact ttataagtca ggctaataac
     1621 agcttccgga ctctagaaca tccctacagg tgatatcctc gggtaacttg tttattgcag
     1681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtcgtc
     1801 gaattcgagt tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     1861 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     1921 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     1981 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     2041 gacgtaatta catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg
     2101 cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
     2161 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     2221 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     2281 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     2341 gggggctttg ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtacctcac
     2401 ccgattctca tcatggagac acatcaggac atctccaata ccgccatgga aacgcaccaa
     2461 gatgcgactg ctcttctgcc agtgacggca ttcgtctgca agattaacgg catacagaaa
     2521 gaaacgatga gacccattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
     2581 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
     2641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
     2761 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3001 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3181 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     3241 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
     3301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3361 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
     3421 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
     3481 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3721 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3781 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
     3841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3961 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4141 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     4201 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
     4261 cctccggtcg gaggcttttg a
//
