LOCUS       sequence-273582-       12054 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses EGFP-tagged nuclear envelope marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
FEATURES             Location/Qualifiers
     source          1..12054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..1188
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          237..1179
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1136..1156
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1243..1252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1249..1251,1252..1254,1255..1965)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1294..1315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1555..1574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1902..1923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4047..4621,4622..4624,4625..4633)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4214..4231)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4441..4460
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4659..5678
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4659..5678
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    5692..6280
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5745..5765)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6163..6174)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6487..7120
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7248..7264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7248..7264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7261..7283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7272..7288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7296..7302,7303..7320,7321..7326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7341..7362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7479..7496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7650..8238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7730..7749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8409..9200,9201..9269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9032..9051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9270..9374)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9422..9556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9459..9478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9513..9532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9724..10357
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10404..10529
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11026..11259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11444..11488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11751..11866
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      361 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      421 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      481 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      541 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
      601 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
      661 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
      721 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
      781 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
      841 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
      901 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
      961 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1021 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1081 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1141 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg atctatttcc
     1201 ggtgaattcc tcgagactag ttctagagcg gccgcaccgg tcgccaccat ggtgagcaag
     1261 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1321 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1381 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1441 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1501 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1561 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1621 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1681 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1741 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1861 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
     1981 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcagcgctgc caacctgccg
     2041 gccatggaga ccccgtccca gcggcgcgcc acccgcagcg gggcgcaggc cagctccact
     2101 ccgctgtcgc ccacccgcat cacccggctg caggagaagg aggacctgca ggagctcaat
     2161 gatcgcttgg cggtctacat cgaccgtgtg cgctcgctgg aaacggagaa cgcagggctg
     2221 cgccttcgca tcaccgagtc tgaagaggtg gtcagccgcg aggtgtccgg catcaaggcc
     2281 gcctacgagg ccgagctcgg ggatgcccgc aagacccttg actcagtagc caaggagcgc
     2341 gcccgcctgc agctggagct gagcaaagtg cgtgaggagt ttaaggagct gaaagcgcgc
     2401 aataccaaga aggagggtga cctgatagct gctcaggctc ggctgaagga cctggaggct
     2461 ctgctgaact ccaaggaggc cgcactgagc actgctctca gtgagaagcg cacgctggag
     2521 ggcgagctgc atgatctgcg gggccaggtg gccaagcttg aggcagccct aggtgaggcc
     2581 aagaagcaac ttcaggatga gatgctgcgg cgggtggatg ctgagaacag gctgcagacc
     2641 atgaaggagg aactggactt ccagaagaac atctacagtg aggagctgcg tgagaccaag
     2701 cgccgtcatg agacccgact ggtggagatt gacaatggga agcagcgtga gtttgagagc
     2761 cggctggcgg atgcgctgca ggaactgcgg gcccagcatg aggaccaggt ggagcagtat
     2821 aagaaggagc tggagaagac ttattctgcc aagctggaca atgccaggca gtctgctgag
     2881 aggaacagca acctggtggg ggctgcccac gaggagctgc agcagtcgcg catccgcatc
     2941 gacagcctct ctgcccagct cagccagctc cagaagcagc tggcagccaa ggaggcgaag
     3001 cttcgagacc tggaggactc actggcccgt gagcgggaca ccagccggcg gctgctggcg
     3061 gaaaaggagc gggagatggc cgagatgcgg gcaaggatgc agcagcagct ggacgagtac
     3121 caggagcttc tggacatcaa gctggccctg gacatggaga tccacgccta ccgcaagctc
     3181 ttggagggcg aggaggagag gctacgcctg tcccccagcc ctacctcgca gcgcagccgt
     3241 ggccgtgctt cctctcactc atcccagaca cagggtgggg gcagcgtcac caaaaagcgc
     3301 aaactggagt ccactgagag ccgcagcagc ttctcacagc acgcacgcac tagcgggcgc
     3361 gtggccgtgg aggaggtgga tgaggagggc aagtttgtcc ggctgcgcaa caagtccaat
     3421 gaggaccagt ccatgggcaa ttggcagatc aagcgccaga atggagatga tcccttgctg
     3481 acttaccggt tcccaccaaa gttcaccctg aaggctgggc aggtggtgac gatctgggct
     3541 gcaggagctg gggccaccca cagcccccct accgacctgg tgtggaaggc acagaacacc
     3601 tggggctgcg ggaacagcct gcgtacggct ctcatcaact ccactgggga agaagtggcc
     3661 atgcgcaagc tggtgcgctc agtgactgtg gttgaggacg acgaggatga ggatggagat
     3721 gacctgctcc atcaccacca cggctcccac tgcagcagct cgggggaccc cgctgagtac
     3781 aacctgcgct cgcgcaccgt gctgtgcggg acctgcgggc agcctgccga caaggcatct
     3841 gccagcggct caggagccca ggtgggcgga cccatctcct ctggctcttc tgcctccagt
     3901 gtcacggtca ctcgcagcta ccgcagtgtg gggggcagtg ggggtggcag cttcggggac
     3961 aatctggtca cccgctccta cctcctgggc aactccagcc cccgaaccca gagcccccag
     4021 aactgcagca tcatgtaagg atcccgcccc tctccctccc ccccccctaa cgttactggc
     4081 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     4141 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     4201 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     4261 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     4321 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     4381 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     4441 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4501 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4561 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4621 gcttgccaca acccgtacca aagatggata gatccggaaa gcctgaactc accgcgacgt
     4681 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     4741 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     4801 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     4861 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
     4921 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     4981 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     5041 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     5101 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     5161 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     5221 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     5281 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     5341 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     5401 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     5461 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     5521 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     5581 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     5641 accgacgccc cagcactcgt ccgagggcaa aggaatagac gcgtctggaa caatcaacct
     5701 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5761 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5821 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5881 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5941 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6001 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6061 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6121 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6181 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6241 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
     6301 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     6361 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     6421 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     6481 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     6541 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     6601 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     6661 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     6721 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     6781 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     6841 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     6901 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     6961 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7021 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7081 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7141 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     7201 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7261 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7321 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7381 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7441 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7501 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7561 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     9421 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9541 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     9601 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     9661 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     9721 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     9781 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     9841 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     9901 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     9961 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    10021 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
    10081 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
    10141 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
    10201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10321 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    10381 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    10441 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    10501 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    10561 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    10621 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    10681 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    10741 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    10801 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    10861 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    10921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    11041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    11101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    11161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    11221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    11281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    11341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    11401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    11521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    11581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    11641 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    11701 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc tttaaaagaa
    11761 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
    11821 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    11881 gggacagcag agatccagtt tatcgatgag taattcatac aaaaggactc gcccctgcct
    11941 tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga gatcgctgcg
    12001 ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag catg
//
