LOCUS       sequence-275014-        3870 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For histochemical _-glucuronidase activity assays. This GUS CDS does
            not have an intron..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS_GUS
FEATURES             Location/Qualifiers
     source          1..3870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     64..81
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(105..162)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             177..1988
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      2008..2051
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2055..2073)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2235..2894
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2301..2320)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2915..3009
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3192..3780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3681..3700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
       61 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
      121 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcaggg tctcgaatgt
      181 tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca
      241 gtctggatcg cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa
      301 gccgggcaat tgctgtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta
      361 attatgcggg caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc
      421 agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc
      481 aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg
      541 ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga
      601 ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc
      661 atgatttctt taactatgcc gggatccatc gcagcgtaat gctctacacc acgccgaaca
      721 cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg
      781 ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat gcggatcaac
      841 aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat ccgcacctct
      901 ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt
      961 gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc
     1021 tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat gcggacttgc
     1081 gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg gactggattg
     1141 gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag
     1201 atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac ctctctttag
     1261 gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag gcagtcaacg
     1321 gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc
     1381 acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac
     1441 gggaatattt cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca
     1501 cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg
     1561 tgctgtgcct gaaccgttat tacggatggt atgtccaaag cggcgatttg gaaacggcag
     1621 agaaggtact ggaaaaagaa cttctggcct ggcaggagaa actgcatcag ccgattatca
     1681 tcaccgaata cggcgtggat acgttagccg ggctgcactc aatgtacacc gacatgtgga
     1741 gtgaagagta tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg
     1801 ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc
     1861 gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg caaaccgaag tcggcggctt
     1921 ttctgctgca aaaacgctgg actggcatga acttcggtga aaaaccgcag cagggaggca
     1981 aacaatgagc tttgagaccg aattccagaa atcatcctta gcgaaagcta aggatttttt
     2041 ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2101 ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt tccaactttc
     2161 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     2221 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     2281 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     2341 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     2401 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     2461 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     2521 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     2581 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     2641 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     2701 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     2761 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     2821 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     2881 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
     2941 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     3001 ggtgagaatc caagcctcga gctgtcagac caagtttact catatatact ttagattgat
     3061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctgtggaaa
     3781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3841 ttctttcctg cgttatcccc tgattctgtg
//
