LOCUS       sequence-275818-       10285 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dox-inducible polycistronic reprogramming lentiviral vector 
            expressing mouse Oct4, Klf4, cMyc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002."
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-OKM
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3809
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5280..5339
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    6727..7315
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6780..6800)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7198..7209)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7463..7643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7690..7708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7776..7880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7881..7949,7950..8741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8099..8118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8912..9500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9654..9671
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9911..9932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9947..9952,9953..9970,9971..9977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9985..10001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9990..10012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10009..10025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10009..10025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10075..10094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggct ccggcgaggg caggggaagt cttctaacat
     3781 gcggggacgt ggaggaaaat cccggcccac tcgagatgag gcagccacct ggcgagtctg
     3841 acatggctgt cagcgacgct ctgctcccgt ccttctccac gttcgcgtcc ggcccggcgg
     3901 gaagggagaa gacactgcgt ccagcaggtg ccccgactaa ccgttggcgt gaggaactct
     3961 ctcacatgaa gcgacttccc ccacttcccg gccgccccta cgacctggcg gcgacggtgg
     4021 ccacagacct ggagagtggc ggagctggtg cagcttgcag cagtaacaac ccggccctcc
     4081 tagcccggag ggagaccgag gagttcaacg acctcctgga cctagacttt atcctttcca
     4141 actcgctaac ccaccaggaa tcggtggccg ccaccgtgac cacctcggcg tcagcttcat
     4201 cctcgtcttc cccagcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcagct
     4261 atccgatccg ggccgggggt gacccgggcg tggctgccag caacacaggt ggagggctcc
     4321 tctacagccg agaatctgcg ccacctccca cggccccctt caacctggcg gacatcaatg
     4381 acgtgagccc ctcgggcggc ttcgtggctg agctcctgcg gccggagttg gacccagtat
     4441 acattccgcc acagcagcct cagccgccag gtggcgggct gatgggcaag tttgtgctga
     4501 aggcgtctct gaccacccct ggcagcgagt acagcagccc ttcggtcatc agtgttagca
     4561 aaggaagccc agacggcagc caccccgtgg tagtggcgcc ctacagcggt ggcccgccgc
     4621 gcatgtgccc caagattaag caagaggcgg tcccgtcctg cacggtcagc cggtccctag
     4681 aggcccattt gagcgctgga ccccagctca gcaacggcca ccggcccaac acacacgact
     4741 tccccctggg gcggcagctc cccaccagga ctacccctac actgagtccc gaggaactgc
     4801 tgaacagcag ggactgtcac cctggcctgc ctcttccccc aggattccat ccccatccgg
     4861 ggcccaacta ccctcctttc ctgccagacc agatgcagtc acaagtcccc tctctccatt
     4921 atcaagagct catgccaccg ggttcctgcc tgccagagga gcccaagcca aagaggggaa
     4981 gaaggtcgtg gccccggaaa agaacagcca cccacacttg tgactatgca ggctgtggca
     5041 aaacctatac caagagttct catctcaagg cacacctgcg aactcacaca ggcgagaaac
     5101 cttaccactg tgactgggac ggctgtgggt ggaaattcgc ccgctccgat gaactgacca
     5161 ggcactaccg caaacacaca gggcaccggc cctttcagtg ccagaagtgc gacagggcct
     5221 tttccaggtc ggaccacctt gccttacaca tgaagaggca ctttctcgag ggctcgggcc
     5281 agtgtactaa ttatgctctc ttgaaattgg ctggagatgt tgagagcaac ccaggtcccg
     5341 gcgcgccact ggatttcctt tgggcgttgg aaaccccgca gacagccacg acgatgcccc
     5401 tcaacgtgaa cttcaccaac aggaactatg acctcgacta cgactccgta cagccctatt
     5461 tcatctgcga cgaggaagag aatttctatc accagcaaca gcagagcgag ctgcagccgc
     5521 ccgcgcccag tgaggatatc tggaagaaat tcgagctgct tcccaccccg cccctgtccc
     5581 cgagccgccg ctccgggctc tgctctccat cctatgttgc ggtcgctacg tccttctccc
     5641 caagggaaga cgatgacggc ggcggtggca acttctccac cgccgatcag ctggagatga
     5701 tgaccgagtt acttggagga gacatggtga accagagctt catctgcgat cctgacgacg
     5761 agaccttcat caagaacatc atcatccagg actgtatgtg gagcggtttc tcagccgctg
     5821 ccaagctggt ctcggagaag ctggcctcct accaggctgc gcgcaaagac agcaccagcc
     5881 tgagccccgc ccgcgggcac agcgtctgct ccacctccag cctgtacctg caggacctca
     5941 ccgccgccgc gtccgagtgc attgacccct cagtggtctt tccctacccg ctcaacgaca
     6001 gcagctcgcc caaatcctgt acctcgtccg attccacggc cttctctcct tcctcggact
     6061 cgctgctgtc ctccgagtcc tccccacggg ccagccctga gcccctagtg ctgcatgagg
     6121 agacaccgcc caccaccagc agcgactctg aagaagagca agaagatgag gaagaaattg
     6181 atgtggtgtc tgtggagaag aggcaaaccc ctgccaagag gtcggagtcg ggctcatctc
     6241 catcccgagg ccacagcaaa cctccgcaca gcccactggt cctcaagagg tgccacgtct
     6301 ccactcacca gcacaactac gccgcacccc cctccacaag gaaggactat ccagctgcca
     6361 agagggccaa gttggacagt ggcagggtcc tgaagcagat cagcaacaac cgcaagtgct
     6421 ccagccccag gtcctcagac acggaggaaa acgacaagag gcggacacac aacgtcttgg
     6481 aacgtcagag gaggaacgag ctgaagcgca gcttttttgc cctgcgtgac cagatccctg
     6541 aattggaaaa caacgaaaag gcccccaagg tagtgatcct caaaaaagcc accgcctaca
     6601 tcctgtccat tcaagcagac gagcacaagc tcacctctga aaaggactta ttgaggaaac
     6661 gacgagaaca gttgaaacac aaactcgaac agcttcgaaa ctctggtgca taaatcgata
     6721 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6781 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6841 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6901 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6961 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7021 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7081 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7141 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7201 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7261 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     7321 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7381 ggggggactg gaagggctaa ttcactccca acgaagacaa gatcacctgc aggacaggcg
     7441 cgccctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7621 gtcagtgtgg aaaatctcta gcacccgggc gattaaggaa agggctagat cattcttgaa
     7681 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7741 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     8161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8401 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8761 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8821 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8881 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8941 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9001 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9061 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9121 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9181 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9241 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9301 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9361 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9421 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9481 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9541 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9601 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9661 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9721 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
     9781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9841 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9901 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9961 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10021 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10081 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    10141 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    10201 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10261 aaatatgtac tacaaactta gtagt
//
