LOCUS       sequence-27586-e         716 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Kaelin WG, Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM
  TITLE     Identification of cellular proteins that can interact specifically 
            with the T/E1A-binding region of the retinoblastoma gene product.
  JOURNAL   Cell. 1991 Feb 8. 64(3):521-32.
  PUBMED    1825028
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1991 
            Feb 8. 64(3):521-32."
COMMENT     Sequence Label: Rb-379-928-Delta-738-775-pGex2T
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(229..251)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(396..414)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcttnattt gaaggatcag atgaagcaga tggaagtaaa catctcccag gagagtccaa
       61 atttcagcag aaactggcag aaatgacttc tactcgaaca cgaatgcaaa agcagaaaat
      121 gaatgatagc atggatacct caaacaagga agagaaatga ggatccccgg gaattcatcg
      181 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
      361 cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      421 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcatttgcn cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga aacgcagttg ggtgcacnan tnggnt
//
