LOCUS       sequence-275931-        5712 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments 
            of mouse Cd36 enhancers at Chr5:17876439_17877752.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Kuang Z, Wang Y, Li Y, Ye C, Ruhn KA, Behrendt CL, Olson EN, Hooper 
            LV
  TITLE     The intestinal microbiota programs diurnal rhythms in host 
            metabolism through histone deacetylase 3.
  JOURNAL   Science. 2019 Sep 27;365(6460):1428-1434. doi: 
            10.1126/science.aaw3134.
  PUBMED    31604271
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-09-27- 27"; volume: "365"; 
            issue: "6460"; pages: "1428-1434"
COMMENT     Sequence Label: pGL4.24-Cd36-enhancer4
FEATURES             Location/Qualifiers
     source          1..5712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..32
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..1714
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1718..1837
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1889..2010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1900..1919)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1954..1973
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2258..2275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2429..3017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2509..2528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3217..4008,4009..4077))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4182..4230
                     /note="synthetic polyadenylation signal"
     misc_feature    4244..4335
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4284..4303
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
       61 caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga
      121 cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac
      181 catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat
      241 gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt
      301 ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg
      361 tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg
      421 catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt
      481 gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca
      541 gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta
      601 cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag
      661 tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt
      721 cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag
      781 cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg
      841 ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga
      901 ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac
      961 tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct
     1021 cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg
     1081 ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc
     1141 tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg
     1201 taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag
     1261 cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca
     1321 cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa
     1381 gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct
     1441 gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga
     1501 gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt
     1561 ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt
     1621 ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct
     1681 cattaaggcc aagaagggcg gcaagatcgc cgtgaattct cacggcttcc ctcccgaggt
     1741 ggaggagcag gccgccggca ccctgcccat gagctgcgcc caggagagcg gcatggatag
     1801 acaccctgct gcttgcgcca gcgccaggat caacgtctaa ggccgcgact ctagagtcgg
     1861 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     1921 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1981 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2041 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2101 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     2161 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     2221 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3181 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     3241 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     3301 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     3361 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     3421 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     3481 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     3541 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     3601 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     3661 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     3721 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     3781 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     3841 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     3901 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     3961 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     4021 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     4081 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     4141 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     4201 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     4261 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     4321 ccagaacatt tctctggcct aactggccgg tacccactgc ttcccctttg agaggttgta
     4381 gaggtttgaa tctgtttcca gatcccagat gatgtaaaaa agaatggtgg atatcccttg
     4441 cttattggtc tctggccttg ggaaggcact ttgtctcttg actttttctc ccaactctta
     4501 cctcttctat cttccactca agacccccca cttgacctta tatttatatt aagttatatt
     4561 tgagacacta aactactatt ctacaacaca ctgaagttaa gctttttgta gctaaatata
     4621 tcactgttat acaaactgag aaaatggaag aattagggtt ctattcatgt cataacacta
     4681 actgaagcct taaagtcacc ttttattatc tccgctgcca ttcagaactg ctccgccaac
     4741 aagcagagct gctggtgtac agtaagtact aagatcgctg ttttgacctt tagtggttta
     4801 tcatctgcct ttactgagac tgttgcttga tttcttatct ggtttccact gcctcttttt
     4861 tctctaccct gcctgttatg tctcaaagta tcctcagaag cagttaggaa aagcatgtgt
     4921 ctcatgtatg ataatggtgt ttgaagtttg ctttttgctt tttcagctct ttctgttgtc
     4981 acattgaacg tcaagcacat tcccaggtaa tccacactcc gtctagacta ggcacgacgt
     5041 acttttctag aactgcctac acatgtcagt tcaggacctt tatgtggttt tggtgttatt
     5101 gtattaatac ttctgggtga ataatcttcc ctattttctc cttttcatat ccccttcagt
     5161 tacaaattcg tatcctgttt aacattcttt acttagaatt acttaatagt gtcattctgc
     5221 tctcagcata aaggtcaaac tccttagaat attgcacaag gcacacaaag ttctggtcag
     5281 cacccaatat gctagcttct tgtcagtttt ccaaatgtgt cactcccttc ctgcttctga
     5341 aatgacacat tccgccccta atatcttttc ttccttttgt ggtactagaa aatgtgtgct
     5401 cacgaattga aacaagttca ttattttagc ctacctcccc tcatcaaacc cagtatcttt
     5461 taaaactatt ttcttttctt tgaattctgg tgcttgttcc ttcagagatt tcttccttct
     5521 taatgtgaaa ttgaacattt tcattccttt ttttataact ctctttctaa aagctttaca
     5581 aaattgcttt ataaaaattc atgatgtttc cccaatggct attttcaaag gtaaaacttg
     5641 catatatgtg gtattgggct gtatttgact cgaggatatc aagatctggc ctcggcggcc
     5701 aagcttagac ac
//
