LOCUS       sequence-276142-         594 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(703-800)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Elias RD, Ma W, Ghirlando R, Schwieters CD, Reddy VS, Deshmukh L
  TITLE     Proline-rich domain of human ALIX contains multiple TSG101-UEV 
            interaction sites and forms phosphorylation-mediated reversible 
            amyloids.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 
            10.1073/pnas.2010635117.
  PUBMED    32917811
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 
            10.1073/pnas.2010635117."
COMMENT     Sequence Label: GB1-PRD(703-800)-Strep
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..183
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             184..195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             235..252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..273
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             568..591
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 caatacaaac tggcgctgaa cggcaaaacg ctgaagggtg aaacgacgac cgaggccgtt
       61 gatgcggcca cggccgagaa agtgttcaag cagtacgcca acgacaacgg tgttgatggc
      121 gaatggacct acgacgatgc gaccaaaacc ttcaccgtga ccgaactggt tccgcgcggc
      181 agtatcgaag gtcgcatccg cgagaatctg aaagatagcg gtctgtttct ggagcatcac
      241 caccatcacc atgaaaatct gtacttccaa ggcgacgaac tgctgaaaga tctgcagcag
      301 agcattgccc gcgaaccaag cgccccaagc atcccgacgc cagcctatca gagtagtccg
      361 gcgggtggtc atgcgccgac cccaccaacg ccggccccac gtacgatgcc gccaaccaaa
      421 ccgcaaccac cagcgcgtcc gccaccacca gttctcccag ccaatcgcgc cccaagtgcg
      481 accgcgccaa gcccagttgg tgcgggtacc gccgcgccag cgccaagtca aacgccgggt
      541 agtgccccac caccacaagc gcaaggctgg agccatccgc agttcgaaaa gtaa
//
