LOCUS       sequence-276274-       13699 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of the centrosomal protein SAS6 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-HsSAS6
FEATURES             Location/Qualifiers
     source          1..13699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2708..4633)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGXGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5068..5086)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5154..5258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5259..5327,5328..6119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5477..5496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6290..6878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7067..7087)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7085..7382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7404..7802
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7846..7980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8118..8137)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8414..8675
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8792..9364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9372..9391
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9419..9438
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9419..9437
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9448..10068
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9521..9540)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10110..10231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10286..10665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10666..10869
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10820..10840
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10871..10889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10892..10910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10920..10944
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11044..11067
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11068..11085
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11635..11658
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11671..11695
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13689..13699,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     3841 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     4021 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     4081 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     4141 ctcctgcccc tcctccysct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4201 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     4261 ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg
     4321 ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg
     4381 tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc
     4441 ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc
     4501 ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc
     4561 cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc
     4621 cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta gacgacgctc
     4681 agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct
     4741 tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg
     4801 tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg
     4861 tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat
     4921 ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg
     4981 gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca
     5041 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5101 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     5821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6421 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6901 tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc
     6961 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     7021 tggggaaggc catccagcct cgcgtcgaac tagatgatcc ggctgtggaa tgtgtgtcag
     7081 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7141 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7201 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7381 ggagggtgac cgccacgacc ggtatggcca agcctttgtc tcaagaagaa tccaccctca
     7441 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7501 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7561 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7621 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7681 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7741 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     7801 aacggtccga cgcggcccga cgggtccgag gggggtcgac ctcgaaactt gtttattgca
     7861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     7981 tgatccgaac cccttcctcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     8041 gcaacgttgt tgccattgct cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     8101 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     8161 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     8221 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     8281 cgcgatgtac gggccagata tacgcgtatc tgaggggact agggtgtgtt taggcgaaaa
     8341 gcggggcttc ggttgtacgc ggttaggagt cccctcagga tatagtagtt tcgcttttgc
     8401 atagggaggg ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8461 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     8521 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8581 gaaccactga attccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa
     8641 acgccatttg accattcacc acattggtgt gcacctccaa gctggccggg gatcctctag
     8701 ggcctctgag ctattccaga agtagtgaag aggctttttt ggaggcctag gcttttgcaa
     8761 aaagctccgg atcgatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt
     8821 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt
     8881 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca
     8941 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt
     9001 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc
     9061 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg
     9121 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct
     9181 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt
     9241 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa
     9301 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct
     9361 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgta
     9421 atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa agtgattaac
     9481 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc
     9541 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg
     9601 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa
     9661 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta
     9721 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa
     9781 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta
     9841 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat
     9901 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga
     9961 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa
    10021 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc gtacagcgga
    10081 tcccgggaat tcagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
    10141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgaatcca tggcgcagaa
    10261 ctggtaggta tggaagatcc gcgttgacat tgattattga ctagttatta atagtaatca
    10321 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    10381 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    10441 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10501 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10561 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10621 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
    10681 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
    10741 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
    10801 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
    10861 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
    10921 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
    10981 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttaccacc
    11041 atggattata aagacgatga tgacaagcac caccaccacc accacgacaa ggattgtgaa
    11101 atgaaacgca ccacactgga cagccctttg gggaagctgg agctgtctgg ttgtgagcag
    11161 ggtctgcacg aaataatttt tctgggcaag gggacgtctg cagctgatgc cgtggaggtc
    11221 ccagcccccg ctgcggttct cggaggtccg gagcccctga tacaggctac agcctggctg
    11281 aatgcctatt tccaccagcc cgaggctatc gaagagttcc ccgtgccggc tcttcaccat
    11341 cccgttttcc agcaagagtc gttcaccaga caggtgttat ggaagctgct gaaggttgtg
    11401 aaattcggag aagtgatttc tgagtcacac ttagcagccc tggtaggcaa ccccgcagcc
    11461 acggcagcag tgaatacggc actggatggc aatcctgtcc ctatcctgat cccgtgccac
    11521 agagtggtcc agggggattc ggatgtgggg ccgtaccttg gtggactggc cgtgaaggaa
    11581 tggcttctgg cccatgaagg ccaccggttg gggaagccag gcttgggatt cgaactggag
    11641 gtgctgttcc agggccccgg taccgccatc acaagtttgt acaaaaaagc aggctttatc
    11701 accggtacca tgagccaagt gctgttccac caactagtcc cgttgcaggt gaaatgcaaa
    11761 gactgtgagg agaggagagt aagtataaga atgagcattg aactacaatc agtttctaat
    11821 ccagttcaca gaaaggactt agttattcgt ctgactgatg acacggatcc atttttttta
    11881 tataaccttg ttatatctga ggaagatttt caaagtttaa aattccagca aggtcttctg
    11941 gtagacttct tagctttccc acaaaaattt atagatctcc ttcagcaatg tactcaagaa
    12001 catgccaaag aaattccaag gtttttgcta cagttagttt ctccagcagc tattttggat
    12061 aactcacctg catttttaaa tgtggtagag acaaatcctt ttaagcatct tacacacctc
    12121 tcactaaaac ttttacctgg aaatgatgtg gagataaaga aatttctcgc aggctgtttg
    12181 aaatgtagca aggaagaaaa attatcattg atgcaatcac tagatgatgc tactaagcaa
    12241 ctggacttta cacgaaagac attagcagaa aaaaaacaag agctcgataa gttacggaat
    12301 gaatgggcgt cacatacagc agccttgaca aacaagcatt ctcaggaact gacaaatgaa
    12361 aaggaaaaag ccttgcaggc acaggttcaa tatcaacagc agcatgaaca acagaaaaaa
    12421 gatttagaaa tcctccatca acaaaacatc caccagctac aaaacagact gtctgagtta
    12481 gaagcggcta ataaagactt aaccgaaaga aaatataaag gagactccac tattagagaa
    12541 cttaaagcaa aactttctgg tgttgaagag gagctacagc ggactaagca agaagtcctc
    12601 tctttgcgaa gagagaattc tacactagat gttgaatgcc acgagaaaga aaagcacgtt
    12661 aatcagctac aaacaaaagt ggcagtttta gaacaggaaa tcaaggataa ggaccagctt
    12721 gttttaagaa caaaagaggc atttgataca atccaggaac aaaaggtggt tttagaagaa
    12781 aatggtgaga aaaatcaagt acaactagga aagcttgaag ctacaataaa atcattatct
    12841 gcagaacttc tgaaggcaaa tgaaattatc aagaagttac aaggggatct gaaaacttta
    12901 atgggtaagt tgaaattgaa gaatacagtt actattcagc aagaaaaact cttggctgag
    12961 aaggaggaaa aattacaaaa ggaacaaaag gaattacaag atgttggaca gtctcttcga
    13021 attaaagagc aagaggtatg caaattacaa gaacaattag aagctacagt taaaaaactt
    13081 gaagaaagca aacaacttct aaaaaataat gaaaagttaa tcacgtggtt aaataaagaa
    13141 ctaaatgaaa atcagctagt gagaaagcaa gatgtattgg gaccttctac tactccgcct
    13201 gcacattcca gcagcaacac aatcagaagt ggaatttctc ctaacctgaa tgtggttgat
    13261 ggtagactga cttacccaac ctgtgggatt ggttatcctg tctcctctgc atttgcattc
    13321 cagaatacct tccctcattc gatatctgcc aaaaatacca gccaccctgg ttcaggaaca
    13381 aaggttcagt ttaatttgca gtttacaaaa ccaaatgcat cactaggaga tgttcagtca
    13441 ggagcaacta ttagtatgcc ttgctcaact gataaggaaa atggtgaaaa tgtagggttg
    13501 gaatccaaat acctgaagaa aagggaagat agcattcctt tacgcggact cagccagaac
    13561 ctatttagta attcagacca tcagagagat ggcactttag gagcattaca tacatcttcc
    13621 aaacccacag cgctcccctc tgcgtcttca gcctatttcc ctgggcagtt accaaacagt
    13681 taatctagac ccagctttc
//
