LOCUS       sequence-276322-       13269 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression cassette for phiC31 Integrase in T-DNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Cody JP, Graham ND, Zhao C, Swyers NC, Birchler JA
  TITLE     Site-specific recombinase genome engineering toolkit in maize.
  JOURNAL   Plant Direct. 2020 Mar 9;4(3):e00209. doi: 10.1002/pld3.209. 
            eCollection 2020 Mar.
  PUBMED    32166212
REFERENCE   2  (bases 1 to 13269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-03-9"; volume: "4(3):e00209. doi"; pages: " 
            10.1002/pld3.209. eCollection 2020 Mar"
COMMENT     Sequence Label: pTFPhiC31
FEATURES             Location/Qualifiers
     source          1..13269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3181..3201
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3232..3484
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3548..3564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3548..3564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3561..3583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3572..3588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3596..3602,3603..3620,3621..3626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3641..3662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3820..3844
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5175..5804
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6233..7306
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7372..7566
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7802..7824)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7910..8050
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7965..7984
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8065..8082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8236..8824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8316..8335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9070..9861)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10382..10406
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     10606..10628
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10620..10637
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10621..10637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(11207..11755)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(11907..12251)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(11943..11963)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt
       61 acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca
      121 tggttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta
      181 gatccgtgct gctagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg
      241 ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg
      301 ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt
      361 atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt
      421 ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca
      481 aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt
      541 tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg
      601 gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg
      661 gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg
      721 tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag
      781 atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat
      841 atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta
      901 taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg
      961 cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac
     1021 tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcagga caatggacac
     1081 gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgag aattcgagcg cagcaagccc
     1141 agcgacacag cgtagcgcca acgaagacaa ggcggccgac cttcagcgcg aagtcgagcg
     1201 cgacgggggc cggttcaggt aagttgttca tggtgccatt agggccactc tttctgatgt
     1261 gagctttaga aagttgatga tttgtccttg catctgcata aagattcggt ttctcagtga
     1321 ttgcacctaa ttcccagata tgtccctagt tgtgagttgt gctctccttt tggaggacta
     1381 tgatagtgtt atggtagtta acttctgaga cgtgtctgta aatgttttag aaaaagtttg
     1441 tgtgtgagct tcatattttg gtaactctgg tataaactag tctgatgcat tgaatatttc
     1501 attatgttta ggttcgtcgg gcatttcagc gaagcgccgg gcacgtcggc gttcgggacg
     1561 gcggagcgcc cggagttcga acgcatcctg aacgaatgcc gcgccgggcg gctcaacatg
     1621 atcattgtct atgacgtgtc gcgcttctcg cgcctgaagg tcatggacgc gattccgatt
     1681 gtctcggaat tgctcgccct gggcgtgacg attgtttcca ctcaggaagg cgtcttccgg
     1741 cagggaaacg tcatggacct gattcacctg attatgcggc tcgacgcgtc gcacaaagaa
     1801 tcttcgctga agtcggcgaa gattctcgac acgaagaacc ttcagcgcga attgggcggg
     1861 tacgtcggcg ggaaggcgcc ttacggcttc gagcttgttt cggagacgaa ggagatcacg
     1921 cgcaacggcc gaatggtcaa tgtcgtcatc aacaagcttg cgcactcgac cactcccctt
     1981 accggaccct tcgagttcga gcccgacgta atccggtggt ggtggcgtga gatcaagacg
     2041 cacaaacacc ttcccttcaa gccgggcagt caagccgcca ttcacccggg cagcatcacg
     2101 gggctttgta agcgcatgga cgctgacgcc gtgccgaccc ggggcgagac gattgggaag
     2161 aagaccgctt caagcgcctg ggacccggca accgttatgc gaatccttcg ggacccgcgt
     2221 attgcgggct tcgccgctga ggtgatctac aagaagaagc cggacggcac gccgaccacg
     2281 aagattgagg gttaccgcat tcagcgcgac ccgatcacgc tccggccggt cgagcttgat
     2341 tgcggaccga tcatcgagcc cgctgagtgg tatgagcttc aggcgtggtt ggacggcagg
     2401 gggcgcggca aggggctttc ccgggggcaa gccattctgt ccgccatgga caagctgtac
     2461 tgcgagtgtg gcgccgtcat gacttcgaag cgcggggaag aatcgatcaa ggactcttac
     2521 cgctgccgtc gccggaaggt ggtcgacccg tccgcacctg ggcagcacga aggcacgtgc
     2581 aacgtcagca tggcggcact cgacaagttc gttgcggaac gcatcttcaa caagatcagg
     2641 cacgccgaag gcgacgaaga gacgttggcg cttctgtggg aagccgcccg acgcttcggc
     2701 aagctcactg aggcgcctga gaagagcggc gaacgggcga accttgttgc ggagcgcgcc
     2761 gacgccctga acgcccttga agagctgtac gaagaccgcg cggcaggcgc gtacgacgga
     2821 cccgttggca ggaagcactt ccggaagcaa caggcagcgc tgacgctccg gcagcaaggg
     2881 gcggaagagc ggcttgccga acttgaagcc gccgaagccc cgaagcttcc ccttgaccaa
     2941 tggttccccg aagacgccga cgctgacccg accggcccta agtcgtggtg ggggcgcgcg
     3001 tcagtagacg acaagcgcgt gttcgtcggg ctcttcgtag acaagatcgt tgtcacgaag
     3061 tcgactacgg gcagggggca gggaacgccc atcgagaagc gcgcttcgat cacgtgggcg
     3121 aagccgccga ccgacgacga cgaagacgac gcccaggacg gcacggaaga cgtagcggcg
     3181 cctaagaaga agaggaaggt ttaggatcca ccggagatcc ctggccggcc cgatcgttca
     3241 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     3301 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     3361 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     3421 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     3481 gatccgatga taagctgtca aacatgagaa ttgggcccag gggtaccgag ctcgaattcg
     3541 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3721 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggagcttga gcttggatca
     3781 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     3841 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     3901 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3961 tactttaaag tactttaaag tactttaaag tactttgatc caacccctcc gctgctatag
     4021 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     4081 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     4141 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     4201 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     4261 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     4321 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     4381 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     4441 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     4501 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     4561 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     4621 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     4681 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     4741 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     4801 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     4861 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     4921 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     4981 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     5041 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     5101 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     5161 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     5221 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     5281 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     5341 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     5401 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     5461 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     5521 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     5581 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     5641 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     5701 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     5761 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     5821 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     5881 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     5941 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     6001 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     6061 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     6121 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     6181 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     6241 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     6301 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     6361 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     6421 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     6481 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     6541 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     6601 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     6661 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     6721 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     6781 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     6841 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     6901 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     6961 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     7021 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     7081 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     7141 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     7201 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     7261 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     7321 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag
     7381 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     7441 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     7501 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     7561 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     7621 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     7681 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     7741 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     7801 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7861 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7921 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     7981 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8041 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8641 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8881 ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt attatgcacg
     8941 cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat tatgtgctta
     9001 gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga acgaatttct
     9061 agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     9121 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     9181 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     9241 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     9301 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     9361 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     9421 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     9481 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
     9541 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     9601 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     9661 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     9721 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     9781 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     9841 ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     9901 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     9961 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
    10021 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
    10081 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcccg
    10141 aattgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10201 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10261 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10321 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    10381 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    10441 ggacgttttt aatgtactga attaacgccg aattgctcta gcattcgcca ttcaggctgc
    10501 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    10561 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10621 gtaaaacgac ggccagtgcc aagctaattc gcttcaagac gtgctcaaat cactatttcc
    10681 acacccctat atttctattg cactcccttt taactgtttt ttattacaaa aatgccctgg
    10741 aaaatgcact ccctttttgt gtttgttttt ttgtgaaacg atgttgtcag gtaatttatt
    10801 tgtcagtcta ctatggtggc ccattatatt aatagcaact gtcggtccaa tagacgacgt
    10861 cgattttctg catttgttta accacgtgga ttttatgaca ttttatatta gttaatttgt
    10921 aaaacctacc caattaaaga cctcatatgt tctaaagact aatacttaat gataacaatt
    10981 ttcttttagt gaagaaaggg ataattagta aatatggaac aagggcagaa gatttattaa
    11041 agccgcgtaa gagacaacaa gtaggtacgt ggagtgtctt aggtgactta cccacataac
    11101 ataaagtgac attaacaaac atagctaatg ctcctatttg aatagtgcat atcagcatac
    11161 cttattacat atagatagga gcaaactcta gctagattgt tgagcagatc tcggtgacgg
    11221 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
    11281 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
    11341 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
    11401 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
    11461 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
    11521 ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
    11581 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
    11641 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
    11701 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag
    11761 atcccccgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta aaagaaatga
    11821 tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt gttttgtata
    11881 tgttgtgttg agaattaatt ctcgaggtcc tctccaaatg aaatgaactt ccttatatag
    11941 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    12001 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    12061 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
    12121 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
    12181 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
    12241 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
    12301 caagtgtgtc gtgctccacc atgttgaccg gcatgcaagc ttgcatgcct gcaggtcgac
    12361 tctagaggat ccccctaggt tcgaaggcgc gcattgcatg tctaagttat aaaaaattac
    12421 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
    12481 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
    12541 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
    12601 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
    12661 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
    12721 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
    12781 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
    12841 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
    12901 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
    12961 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
    13021 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
    13081 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
    13141 ctcctctcac ggcaccggca gctacggggg attcctttcc caccgctcct tcgctttccc
    13201 ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt
    13261 tgttcggag
//
