LOCUS       sequence-276427-         812 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike)
            fused with sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pcDNA3-SARS-CoV-2-S-RBD-sfGFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..718)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             complement(8..718)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(48..68)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     663..691
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttatcattt gtagagctca tccatgccat gtgtaatccc agcagcagtt acaaactcaa
       61 gaaggaccat gtggtcacgc ttttcgttgg gatctttcga aaggacagat tgtgtcgaca
      121 ggtaatggtt gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat
      181 ggtctgctag ttgaacggaa ccatcttcaa cgttgtggcg aattttgaag ttagctttga
      241 ttccattctt ttgtttgtct gccgtgatgt atacattgtg tgagttaaag ttgtactcca
      301 gtttgtgtcc gagaatgttt ccatcttctt taaaatcaat accttttaac tcgatacgat
      361 taacaagggt atcaccttca aacttgactt cagcacgcgt cttgtaggtc ccgtcatctt
      421 tgaaagatat agtgcgttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat
      481 gccgtttcat gtgatccgga taacgggaaa agcattgaac accataggtc agagtagtga
      541 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtgagtt
      601 ttccgtttgt agcatcacct tcaccctctc cacggacaga aaatttgtgc ccattaacat
      661 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttggaac
      721 caccactacc ggacccaccg gatccctttt tgggcccaca aactgtagcc ggggcgtgga
      781 ggagctcaaa ggacaatact accactctat aa
//
