LOCUS       sequence-276979-        1394 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing the PBX2 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0422
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgaacg gctactgggg ccgccccctc caggcggggg ccgggggggc
       61 ctgggattgg tgagtgggga gcctgggggc cctggcgagc ctcccggtgg cggagacccc
      121 ggtgggggta gcgggggggt cccgggaggc cgagggaagc aagacatcgg ggacattctg
      181 cagcagataa tgaccatcac cgaccagagc ctggacgagg cccaggccaa gaaacacgcc
      241 ctaaactgcc accgaatgaa gcctgctctc tttagcgtcc tgtgtgaaat caaggagaaa
      301 actggcctca gcattcggag ctcccaggag gaggagccgg tggacccaca gctgatgcgc
      361 ttggacaaca tgcttctggc agagggtgtg gctgggcccg agaaaggggg cggctcagca
      421 gcagcagctg cagccgctgc agcctctggt ggtggtgtgt cccctgacaa ctccatcgaa
      481 cactcggact atcgcagcaa acttgcccag atccgtcaca tataccactc ggagctggag
      541 aagtatgagc aggcatgtaa tgagttcacg acccatgtca tgaacctgct gagggagcag
      601 agccgcacca ggcccgtggc ccccaaagag atggaacgca tggtgagcat catccatcga
      661 aagttcagcg ccatccagat gcagctgaag cagagcacct gcgaggctgt gatgatcctg
      721 cgctcccgtt tcctggatgc cagacgaaag cgccgtaact tcagcaaaca ggccactgag
      781 gtcctaaatg agtatttcta ctcccacctg agtaacccat atcctagtga ggaggccaag
      841 gaggagcttg ccaagaagtg tggcatcacc gtgtctcagg tctccaactg gtttggcaac
      901 aagaggattc gctataagaa aaacatcgga aagttccaag aggaggcaaa catctatgct
      961 gtcaagaccg ccgtgtcagt cacccagggg ggccacagcc gcaccagctc cccgacaccc
     1021 ccttcctctg caggctctgg cggctctttc aatctctcag gatctggaga catgtttctg
     1081 gggatgcctg ggctcaacgg agattcctat tctgcttccc aggtggaatc actccgacac
     1141 tcgatggggc cagggggcta tggggataac ctcgggggag gccagatgta cagcccacgg
     1201 gaaatgaggg caaatggcag ctggcaagag gctgtgaccc cctcttcagt gacatcccca
     1261 acggagggac cagggagtgt tcactctgat acctccaact agtaatgaac tagtccgtaa
     1321 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgatgctgat
     1381 cgtgataggt ggat
//
