LOCUS       sequence-277024-        1148 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing the PAX5 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0519
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttaga gaaaaattat ccgactcctc ggaccagcag gacaggacat
       61 ggaggagtga atcagcttgg gggggttttt gtgaatggac ggccactccc ggatgtagtc
      121 cgccagagga tagtggaact tgctcatcaa ggtgtcaggc cctgcgacat ctccaggcag
      181 cttcgggtca gccatggttg tgtcagcaaa attcttggca ggtattatga gacaggaagc
      241 atcaagcctg gggtaattgg aggatccaaa ccaaaggtcg ccacacccaa agtggtggaa
      301 aaaatcgctg aatataaacg ccaaaatccc accatgtttg cctgggagat cagggaccgg
      361 ctgctggcag agcgggtgtg tgacaatgac accgtgccta gcgtcagttc catcaacagg
      421 atcatccgga caaaagtaca gcagccaccc aaccaaccag tcccagcttc cagtcacagc
      481 ataggtattc aggagtctcc ggtgccgaac ggccactcgc ttccgggcag agacttcctc
      541 cggaagcaga tgcggggaga cttgttcaca cagcagcagc tggaggtgct ggaccgcgtg
      601 tttgagaggc agcactactc agacatcttc accaccacag agcccatcaa gcccgagcag
      661 accacagagt attcagccat ggcctcgctg gctggtgggc tggacgacat gaaggccaat
      721 ctggccagcc ccacccctgc tgacatcggg agcagtgtgc caggcccgca gtcctacccc
      781 attgtgacag gccgtgactt ggcgagcacg accctccccg ggtaccctcc acacgtcccc
      841 cccgctggac agggcagcta ctcagcaccg acgctgacag ggatggtgcc tgggagtgag
      901 ttttccggga gtccctacag ccaccctcag tattcctcgt acaacgactc ctggaggttc
      961 cccaacccgg ggctgcttgg ctccccctac tattatagcg ctgccgcccg aggagccgcc
     1021 ccacctgcag ccgccactgc ctatgaccgt cactagtaat gaactagtcc gtaacttgaa
     1081 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgatta acacgttatc
     1141 tatttaat
//
