LOCUS       sequence-277174-        1427 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0742
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccccaag agcccagatc cagggaccgc tgacatttgg ggatgtggct
       61 gtggccttta cccggattga gtggagacac ctggatgctg ctcagcgggc cctgtacagg
      121 gatgtgatgc tggagaacta tgggaatctg gtgtctgtgg ggcttctctc ttccaaacca
      181 aaactaatta ctcagttgga gcaaggggca gagccctgga ctgaggtgcg agaggctcca
      241 tcaggcacac atgcagtcga ggattactgg tttgaaacaa agatgtcagc cctaaagcaa
      301 agcacttctg aagcatctgt tctgggagag cgaacgaaaa gtgtcatgat ggaaaaaggc
      361 ctggactggg agggcagaag ctccacagag aagaactata agtgcaagga atgtgggaaa
      421 gtcttcaaat acaattcgtc ctttattagc caccagagaa atcacaccag tgagaaacca
      481 cataaatgta aagaatgtgg gatcgccttt atgaacagtt catccctttt aaatcaccat
      541 aaggttcatg caggcaaaca gccttataga tgtattgaat gtgggaagtt cctgaagaag
      601 cactcaacgt ttatcaacca tcagagaatt cattctaggg agaaacccca caaatgcatt
      661 gaatgtggaa aaactttcag aaagaactca atccttttaa gtcatcagag aattcatact
      721 ggccagaaac cctacaaatg taatgactgt gggaaagcct tcgctcagaa tgcagctctt
      781 actcgtcatg aaagaataca tagtggagag aagcctttta aatgtaacaa gtgtgggaga
      841 gctttcaggg ataattcaac tgtgttggaa catcagaaaa tccatactgg tgagaagcca
      901 tatcagtgta atgaatgtgg aaaagccttt aggaagagct caactcttat tagtcaccaa
      961 agaatgcata ctggggagaa accctatcac tgcagtaaat gtggaaaatc tttcaggtat
     1021 agctcatcct ttgctggtca tcagaaaact cacagtggaa ataaaccgta tcagtgtcgt
     1081 gactgtggga aggcctttac aaagagctca acccttactg gacatcagag aattcatact
     1141 ggagaaaaac cctatcactg taagaaatgt gggaaagcct ttcggcacag ctcaggcctt
     1201 gttgaacatc agagactcca tactggggaa aaaccttata aatgtaatga atgtgggaaa
     1261 gcttttcccc gaagttcagc ccttaagcaa cataagaaaa ttcataataa agaaagagcc
     1321 atgaaatgta gttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgagagggca aatcttgcaa atccgct
//
