LOCUS       sequence-277264-        3839 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0804
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggtgcg gaagaaggac ggcggcccca acgtgaagta ctacgaggcc
       61 gcggacaccg tgacccagtt cgacaacgtg cggctgtggc tcggcaagaa ctacaagaag
      121 tatatacaag ctgaaccacc caccaacaag tccctgtcta gcctggttgt acagttgcta
      181 caatttcagg aagaagtttt tggcaaacat gtcagcaatg caccgctcac taaactgccg
      241 atcaaatgtt tcctagattt caaagcggga ggctccttgt gccacattct tgcagctgcc
      301 tacaaattca agagtgacca gggatggcgg cgttacgatt tccagaatcc atcacgcatg
      361 gaccgcaatg tggaaatgtt tatgaccatt gagaagtcct tggtgcagaa taattgcctg
      421 tctcgaccta acatttttct gtgcccagaa attgagccca aactactagg gaaattaaag
      481 gacattatca agagacacca gggaacagtc actgaggata agaacaatgc ctcccatgtt
      541 gtgtatcctg tcccggggaa tctagaagaa gaggaatggg tacgaccagt catgaagagg
      601 gataagcagg ttcttctgca ctggggctac tatcctgaca gttacgacac gtggatccca
      661 gcgagtgaaa ttgaggcatc tgtggaagat gctccaactc ctgagaaacc taggaaggtt
      721 catgcaaagt ggatcctgga caccgacacc ttcaatgaat ggatgaatga ggaagactat
      781 gaagtaaatg atgacaaaaa ccctgtctcc cgccgaaaga agatttcagc caagacactg
      841 acagatgagg tgaacagccc agattcagat cgacgggaca agaagggggg aaactataag
      901 aagaggaagc gctccccctc tccttcacca accccagaag caaagaagaa aaatgctaag
      961 aaaggtccct caacacctta cactaagtca aagcgtggcc acagagaaga ggagcaagaa
     1021 gacctgacaa aggacatgga cgagccctca ccagtcccca atgtagaaga ggtgacactt
     1081 cccaaaacag tcaacacaaa gaaagactca gagtcggccc cagtcaaagg cggcaccatg
     1141 accgacctgg atgaacagga agatgaaagc atggagacga cgggcaagga tgaggatgag
     1201 aacagtacgg ggaacaaggg agagcagacc aagaatccag acctgcatga ggacaatgtg
     1261 actgaacaga cccaccacat catcattccc agctacgctg cctggtttga ctacaatagt
     1321 gttcatgcca ttgagcggag ggctctcccc gagttcttca acggcaagaa caagtccaag
     1381 actccagaga tctacctggc ctatcgaaac tttatgattg acacttaccg actgaacccc
     1441 caagagtatc ttacctctac cgcctgccgc cgaaacctag cgggtgatgt ctgtgccatc
     1501 atgagggtcc atgccttcct agaacagtgg ggtcttatta actaccaggt ggatgctgag
     1561 agtcgaccaa ccccaatggg gcctccgcct acctctcact tccatgtctt ggctgacaca
     1621 ccatcagggc tggtgcctct gcagcccaag acacctcagg gccgccaggt tgatgctgat
     1681 accaaggctg ggcgaaaggg caaagagctg gatgacctgg tgccagagac ggctaagggc
     1741 aagccagagc tgcagacctc tgcttcccaa caaatgctca actttcctga caaaggcaaa
     1801 gagaaaccaa cagacatgca aaactttggg ctgcgcacag acatgtacac aaaaaagaat
     1861 gttccctcca agagcaaggc tgcagccagt gccactcgtg agtggacaga acaggaaacc
     1921 ctgcttctcc tggaggcact ggaaatgtac aaagatgact ggaacaaagt gtccgagcat
     1981 gtgggaagcc gcacacagga cgagtgcatc ttgcattttc ttcgtcttcc cattgaagac
     2041 ccatacctgg aggactcaga ggcctcccta ggccccctgg cctaccaacc catccccttc
     2101 agtcagtcgg gcaaccctgt tatgagcact gttgccttcc tggcctctgt cgtcgatccc
     2161 cgagtcgcct ctgctgctgc aaagtcagcc ctagaggagt tctccaaaat gaaggaagag
     2221 gtacccacgg ccttggtgga ggcccatgtt cgaaaagtgg aagaagcagc caaagtaaca
     2281 ggcaaggcgg accctgcctt cggtctggaa agcagtggca ttgcaggaac cacctctgat
     2341 gagcctgagc ggattgagga gagcgggaat gacgaggctc gggtggaagg ccaggccaca
     2401 gatgagaaga aggagcccaa ggaaccccga gaaggagggg gtgctataga ggaggaagca
     2461 aaagagaaaa ccagcgaggc tcccaagaag gatgaggaga aagggaaaga aggcgacagt
     2521 gagaaggagt ccgagaagag tgatggagac ccaatagtcg atcctgagaa ggagaaggag
     2581 ccaaaggaag ggcaggagga agtgctgaag gaagtggtgg agtctgaggg ggaaaggaag
     2641 acaaaggtgg agcgggacat tggcgagggc aacctctcca ccgctgctgc cgccgccctg
     2701 gccgccgccg cagtgaaagc taagcacttg gctgctgttg aggaaaggaa gatcaaatct
     2761 ttggtggccc tgctggtgga gacccagatg aaaaagttgg agatcaaact tcggcacttt
     2821 gaggagctgg agactatcat ggaccgggag cgagaagcac tggagtatca gaggcagcag
     2881 ctcctggccg acagacaagc cttccacatg gagcagctga agtatgcgga gatgagggct
     2941 cggcagcagc acttccaaca gatgcaccaa cagcagcagc agccaccacc agccctgccc
     3001 ccaggctccc agcctatccc cccaacaggg gctgctgggc cacccgcagt ccatggcttg
     3061 gctgtggctc cagcctctgt agtccctgct cctgctggca gtggggcccc tccaggaagt
     3121 ttgggccctt ctgaacagat tgggcaggca gggtcaactg cagggccaca gcagcagcaa
     3181 ccagctggag ccccccagcc tggggcagtc ccaccagggg ttcccccccc tggaccccat
     3241 ggcccctcac cgttccccaa ccaacaaact cctccctcaa tgatgccagg ggcagtgcca
     3301 ggcagcgggc acccaggcgt ggcgggtaat gctcctttgg gtttgccttt tggcatgccg
     3361 cctcctcctc ctcctcctgc tccatccatc atcccatttg gtagtctagc tgactccatc
     3421 agtattaacc tccccgctcc tcctaacctg catgggcatc accaccatct cccgttcgcc
     3481 ccgggcactc tccccccacc taacctgcct gtgtccatgg cgaaccctct acatcctaac
     3541 ctgccggcga ccaccaccat gccatcttcc ttgcctctcg ggccggggct cggatccgcc
     3601 gcagcccaaa gccctgccat tgtggcagct gttcagggca acctcctgcc cagtgccagc
     3661 ccactgccag acccaggcac ccccctgcct ccagacccca cagccccgag cccaggcacg
     3721 gtcacccctg tgccacctcc acagtagtaa tgaactagtc cgtaacttga aagtatttcg
     3781 atttcttggc tttatatatc ttgtggaaag gacgattcag aactctagaa gaatccagc
//
