LOCUS       sequence-277354-        1475 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0859
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..297
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             271..300
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tgacgatgct cctggacgga ggcccgcagt tccctgggct gggagtgggc
       61 agcttcggcg cgccgcgcca ccacgagatg cccaaccgtg agccggcagg catggggctg
      121 aatcccttcg gggactcaac ccacgccgcc gccgccgccg ccgccgccgc tgccttcaag
      181 ctgagccctg ccgcggcgca cgatctatct tcaggccaga gctcggcttt cacgccgcag
      241 ggttcgggct acgccaacgc cctgggccac catcaccacc accatcacca tcatcaccac
      301 accagccagg tgcccagcta cggtggcgct gcctctgccg ccttcaactc aacgcgcgag
      361 tttctgttcc gccagcgcag ctccgggctc agtgaggcgg cctcgggtgg cgggcagcac
      421 gggctcttcg ccggctcggc gagcagcctg catgctccag ctggcatccc cgagccccct
      481 agctacttgc tgtttcccgg gctgcatgag cagggcgctg ggcacccgtc gcccacaggg
      541 cacgtggaca acaaccaggt ccacctgggg ctgcgtgggg agctgttcgg ccgtgctgac
      601 ccataccgcc cagtggccag cccgcgcacg gacccctacg cggccggcgc tcagtttcct
      661 aactacagcc ccatgaacat gaacatggga gtgaacgtgg cggcccacca cgggcccggc
      721 gccttcttcc gttatatgcg gcagcctatc aagcaggagc tgtcgtgcaa gtggatcgac
      781 gaggctcagc tgagccggcc caagaagagc tgcgaccgga ccttcagcac catgcatgag
      841 ctggtgacac atgtcaccat ggagcatgtg gggggcccgg agcagaacaa ccacgtctgc
      901 tactgggagg agtgcccccg ggagggcaag tctttcaagg cgaagtacaa actggtcaac
      961 cacatccgag tgcacacggg cgagaagccc ttcccatgcc ccttcccggg ctgcgggaag
     1021 atctttgccc gttctgagaa cctcaagatc cacaagagga cccacacagg tgagaaacct
     1081 ttcaaatgtg aatttgaagg ctgtgacaga cgctttgcca acagcagcga ccgtaagaag
     1141 cacatgcatg tgcatacctc ggacaagccc tatatctgca aagtgtgcga caagtcctac
     1201 acgcacccga gctccctgcg caaacacatg aagtgttgtc ctgcttggta tccgggacag
     1261 tctctaattc ctgacgaaga acttgatact gacgttggta tgcagcagcc agccctccat
     1321 aacactacct atcctaaatg cagggttaat gccgaaccta ctgtgcaaga aatgatttac
     1381 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1441 ggaaaggacg aacacgatgt aacggacagg tagta
//
