LOCUS       sequence-277615-        1688 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1473
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtggt gccagctgag attgctaaga agcctcgaac gccaacctct
       61 ggtccagtaa tcacgaagct gatctttgca aaaccaatta atagtaaagc agttacagga
      121 cagacaactc aagtttcacc accagttatt gcaggtaggg ttctttcaca gtctactccc
      181 ggaactccat caaagaccat aacaatatct gaaagtggtg ttattggatc aactttaaat
      241 tctacaacac agacaccaaa taaaatagcc atctcacctt tgaaatcgcc aaataaggca
      301 gtgaaatcaa ctgtgcagac catcactgtt ggaggagtga gcacatcaca gtttaagaca
      361 attattcctc tggcaactgc tcccaatgtc cagcagattc aagtgcctgg aagcaagttt
      421 cattatgtcc gacttgttac tgccacatca gccagtagct caacccagcc agttagtcag
      481 aatcccagta caaacactca gcctcttcag caagcaaagc cagtggttgt taatacaacc
      541 ccagtgcgga tgtcagttcc aattgtctca gctcaggctg tcaaacaagt tgttccaaaa
      601 ccaatcaatc caacttcaca aatagtaact actagccagc cacagcaacg gcttatcatg
      661 cctgccacac cactgccaca gatccagccc aacctcacta acctgccacc aggcactgtc
      721 ctggcaccag ctccgggaac agggaatgtg ggttatgcag tgcttccagc tcagtatgtt
      781 actcagctac agcagtcttc atatgtatca atagcaagca actctacctt tactggaaca
      841 tctggtatcc agacccaggc acggcttcca ttcaatggca taatcccatc agagtcggcc
      901 agtcggcccc gaaagccctg taattgtaca aaatcactgt gtttgaaatt gtattgtgat
      961 tgctttgcaa atggtgaatt ttgcaacaac tgcaattgta ctaattgtta caacaatttg
     1021 gaacatgaaa atgaaaggca aaaagcaata aaggcatgcc ttgacagaaa tccagaagcc
     1081 tttaagccta agatagggaa aggaaaggag ggagaatctg atcgacgtca tagcaaaggg
     1141 tgtaattgca aacgatcagg atgtcttaaa aactactgtg aatgctatga ggcaaaaata
     1201 atgtgttcct caatatgcaa atgtattggc tgtaagaatt ttgaagaaag cccggaaagg
     1261 aagacattga tgcatttggc agatgcagct gaagtaaggg tacagcaaca aacagcagcc
     1321 aagacgaagt tatcctctca aatttcagac ttgcttacta ggccaacacc agctttaaat
     1381 agtggaggcg gaaaattgcc atttacattt gtaactaagg aagtagctga agccacatgt
     1441 aattgcctcc ttgcccaggc agagcaggca gacaagaagg gaaaatcaaa ggcagcagcg
     1501 gaacggatga tacttgagga attcggacga tgtttgatga gtgtcatcaa ctctgcagga
     1561 aaggcaaaaa gtgacccttg tgccatgaat tgctagtaat gaactagtcc gtaacttgaa
     1621 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaattata gccaatatgt
     1681 ttgaactc
//
