LOCUS       sequence-277732-        1769 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1670
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccagtc caccgccacc tcccctgatg ggggcaccac gtttgagcac
       61 ctctggagct ctctggaacc agacagcacc tacttcgacc ttccccagtc aagccggggg
      121 aataatgagg tggtgggcgg aacggattcc agcatggacg tcttccacct ggagggcatg
      181 actacatctg tcatggccca gttcaatctg ctgagcagca ccatggacca gatgagcagc
      241 cgcgcggcct cggccagccc ctacacccca gagcacgccg ccagcgtgcc cacccactcg
      301 ccctacgcac aacccagctc caccttcgac accatgtcgc cggcgcctgt catcccctcc
      361 aacaccgact accccggacc ccaccacttt gaggtcactt tccagcagtc cagcacggcc
      421 aagtcagcca cctggacgta ctccccgctc ttgaagaaac tctactgcca gatcgccaag
      481 acatgcccca tccagatcaa ggtgtccacc ccgccacccc caggcaccgc catccgggcc
      541 atgcctgttt acaagaaagc ggagcacgtg accgacgtcg tgaaacgctg ccccaaccac
      601 gagctcggga gggacttcaa cgaaggacag tctgctccag ccagccacct catccgcgtg
      661 gaaggcaata atctctcgca gtatgtggat gaccctgtca ccggcaggca gagcgtcgtg
      721 gtgccctatg agccaccaca ggtggggacg gaattcacca ccatcctgta caacttcatg
      781 tgtaacagca gctgtgtagg gggcatgaac cggcggccca tcctcatcat catcaccctg
      841 gagatgcggg atgggcaggt gctgggccgc cggtcctttg agggccgcat ctgcgcctgt
      901 cctggccgcg accgaaaagc tgatgaggac cactaccggg agcagcaggc cctgaacgag
      961 agctccgcca agaacggggc cgccagcaag cgtgccttca agcagagccc ccctgccgtc
     1021 cccgcccttg gtgccggtgt gaagaagcgg cggcatggag acgaggacac gtactacctt
     1081 caggtgcgag gccgggagaa ctttgagatc ctgatgaagc tgaaagagag cctggagctg
     1141 atggagttgg tgccgcagcc actggtggac tcctatcggc agcagcagca gctcctacag
     1201 aggccgcccc gggatgctca acaaccatgg ccacgcagtg ccagccaacg gcgagatgag
     1261 cagcagccac agcgcccagt ccatggtctc ggggtcccac tgcactccgc caccccccta
     1321 ccacgccgac cccagcctcg tcaggacctg ggggccctga agatccccga gcagtaccgc
     1381 atgaccatct ggcggggcct gcaggacctg aagcagggcc acgactacag caccgcgcag
     1441 cagctgctcc gctctagcaa cgcggccacc atctccatcg gcggctcagg ggaactgcag
     1501 cgccagcggg tcatggaggc cgtgcacttc cgcgtgcgcc acaccatcac catccccaac
     1561 cgcggcggcc caggcggcgg ccctgacgag tgggcggact tcggcttcga cctgcccgac
     1621 tgcaaggccc gcaagcagcc catcaaggag gagttcacgg aggccgagat ccactagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgagttac ggagtgggat agtaatacc
//
