LOCUS       sequence-277915-        1613 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2107
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaaagg tgggaacata caattggaga ttcctgactt cagcaactct
       61 gtcctgagcc atctaaacca gttgcgcatg cagggccgtc tctgtgatat tgtggtcaat
      121 gtgcaaggac aagcttttcg ggctcacaaa gtggtgctgg ctgccagctc cccctatttc
      181 cgggatcaca tgtccttgaa tgagatgagt acagtctcca tttcagtcat caagaaccct
      241 actgtttttg aacagctcct ttctttctgt tacacagggc ggatatgcct gcaactggca
      301 gatatcatca gctacctaac agctgccagt tttctgcaaa tgcagcatat tatagacaaa
      361 tgtacacaga tcctggaggg cattcatttc aaaattaatg tggctgaggt tgaagcagaa
      421 ttaagtcaaa caaggacaaa gcatcaagag agacctccag agtctcacag ggttacacca
      481 aatctcaacc gctcccttag cccacgacat aataccccaa agggaaaccg gcgaggtcag
      541 gttagtgctg tgctggatat cagagagcta agtcctcctg aggagtccac cagccctcag
      601 atcattgaac caagttctga tgtagagagc cgggagccca ttcttcggat caaccgagca
      661 ggacagtggt atgtggagac aggagtggcg gaccgtgggg gtcggagtga tgatgaagtt
      721 agagttcttg gagcagtaca catcaaaact gaaaatctgg aggagtggct tgggcctgag
      781 aatcagcctt ctggagaaga tgggagtagt gcagaggaag taacagccat ggtgattgat
      841 accacaggcc atggttctgt aggacaggaa aattatactt tagggtcttc aggagccaag
      901 gtggctcggc caacaagcag tgaagttgac agatttagcc cctccggcag tgttgttccc
      961 ttgacagaga gacacagagc cagaagtgag tctcctggga gaatggatga gcctaagcaa
     1021 cccagctccc aggtagaaga gtcagcaatg atgggagtaa gtggctatgt ggagtatctc
     1081 cgagagcagg aagtatctga gcggtggttc cggtacaacc ctcgtctcac ctgcatctat
     1141 tgcgccaaat ctttcaacca gaagggaagc ctggaccgcc acatgcgcct acacatgggg
     1201 atcacaccat tcgtctgccg catgtgtggc aagaagtata cccggaaaga tcagctggag
     1261 tatcatatcc gcaagcacac aggcaacaag ccctttcact gtcatgtctg tggcaaaagt
     1321 ttccccttcc aggccatctt gaatcagcac tttcgcaaaa accaccctgg ctgtataccc
     1381 ctggaggggc ctcacagcat ctcccctgaa acaactgtca catctcgagg acaagctgag
     1441 gaagagtcac cttcacagga agagacagtt gctcctgggg aagctgtcca gggctctgtg
     1501 tccaccactg ggccagacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1561 tggctttata tatcttgtgg aaaggacgat gatgggcccg ttagagagcc agt
//
