LOCUS       sequence-278077-        1070 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2232
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggttgcc ggagagaaga ggattggtcc tgcttttaag cctagctgaa
       61 attcttttca agatcatgat tctggaagga ggtggtgtaa tgaatctcaa ccccggcaac
      121 aacctccttc accagccgcc agcctggaca gacagctact ccacgtgcaa tgtttccagt
      181 gggttttttg gaggccagtg gcatgaaatt catcctcagt actggaccaa gtaccaggtg
      241 tgggagtggc tccagcacct cctggacacc aaccagctgg atgccaattg tatccctttc
      301 caagagttcg acatcaacgg cgagcacctc tgcagcatga gtttgcagga gttcacccgg
      361 gcggcaggga cggcggggca gctcctctac agcaacttgc agcatctgaa gtggaacggc
      421 cagtgcagta gtgacctgtt ccagtccaca cacaatgtca ttgtcaagac tgaacaaact
      481 gagccttcca tcatgaacac ctggaaagac gagaactatt tatatgacac caactatggt
      541 agcacagtag atttgttgga cagcaaaact ttctgccggg ctcagatctc catgacaacc
      601 accagtcacc ttcctgttgc agagtcacct gatatgaaaa aggagcaaga cccccctgcc
      661 aagtgccaca ccaaaaagca caacccgaga gggactcact tatgggaatt catccgcgac
      721 atcctcttga acccagacaa gaacccagga ttaataaaat gggaagaccg atctgagggc
      781 gtcttcaggt tcttgaaatc agaggcagtg gctcagctat ggggtaaaaa gaagaacaac
      841 agcagcatga cctatgaaaa gctcagccga gctatgagat attactacaa aagagaaatt
      901 ctggagcgtg tggatggacg aagactggta tataaatttg ggaagaatgc ccgaggatgg
      961 agagaaaatg aaaactagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1021 ctttatatat cttgtggaaa ggacgaagac tgcggtcagg tgcgcaagtc
//
