LOCUS       sequence-278140-        1667 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2264
FEATURES             Location/Qualifiers
     source          1..1667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagacaga tttggctgaa atgcctgaga aaggagctct gtcttcccag
       61 gattctcccc atttccaaga gaagagcaca gaagagggag aagtggctgc tctgcgcctc
      121 acggccagat cccaggaaac agtgacattc aaggatgtgg ccatggactt tacaccagag
      181 gagtggggga agctggatcc tgcacaaagg gatgtgatgc tggagaacta taggaaccta
      241 gtctcacttt ggcttccagt ttccaaacct gagagctaca acttggagaa tggaaaagaa
      301 ccattgaagc ttgagagaaa agcccccaaa agcagctatt cagacatgga gactagacca
      361 cagagcaagg attcaacttc agtgcaagat ttttccaaag cagaatcatg caaagttgca
      421 ataatagaca gactgacacg gaatagtgtc tatgactcta acttggaagc agcccttgaa
      481 tgtgaaaatt ggttagagaa tcagcaagga aatcaggaga gacatttgag agaaatgttc
      541 actcacatga attcactctc tgaggaaaca gaccataagc atgatgtata ctggaaaagc
      601 ttcaatcaga aatctgtcct tatcactgaa gacagagttc ccaaaggatc ttatgccttc
      661 catacacttg aaaaaagctt gaaacaaaaa tcaaacttaa tgaaaaagca gagaacttat
      721 aaagagaaaa aacctcataa atgtaatgat tgtggtgaac tcttcaccta ccattcagtg
      781 cttattcgac accagagagt ccatactgga gagaaaccct atacctgcaa tgaatgtggg
      841 aaatctttta gccacagagc taatttaact aaacaccaga gaactcatac tagaattctc
      901 tttgaatgca gtgaatgcaa gaaaaccttc acagaaagct catcccttgc aacacatcag
      961 agaattcacg ttggagagag accttatgaa tgcaatgaat gtgggaaagg ttttaatcga
     1021 agtacacatc ttgtgcagca tcagttgatt catactggag tgaagcctta tgaatgtaat
     1081 gaatgtgata aagcttttat tcattcatca gcactcatta aacatcaaag aactcatact
     1141 ggagagaaac cttataaatg tcaagaatgt gggaaagcct ttagccattg ctcatcccta
     1201 actaagcatc agagagttca tactggagaa aagccatatg aatgcagtga atgtggaaaa
     1261 acttttagtc agagcacaca tcttgttcaa catcagagaa ttcacactgg agagaaaccc
     1321 tatgagtgta atgaatgtgg gaaaaccttc agccggagct ccaattttgc taaacatcaa
     1381 agaattcata ttggaaagaa accgtacaaa tgtagcgagt gtggaaaagc cttcattcat
     1441 tcatcagctc tcattcaaca tcagagaact cataccggag agaaaccctt tagatgtaat
     1501 gagtgtggga aaagctttaa gtgcagttca tctctcatca gacatcaaag agttcacact
     1561 gaagagcaac cctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1621 tatatatctt gtggaaagga cgagctaata tattctccct gtgctaa
//
