LOCUS       sequence-278185-         566 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2296
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgatgc ccacgagtcg ccctccgaca aaggtggaga gacaggggag
       61 tcggatgaga cggccgctgt gcccggggac ccgggggcta ccgacaccga tggaatccca
      121 gaggaaactg acggagacgc agatgtggac ttgaaagaag ctgcagcgga ggaaggcgag
      181 ctcgagagtc aggatgtctc agatttaaca acagttgaaa gggaagactc atcattactt
      241 aatcctgcag ccaaaaaact gaaaatagat accaaagaaa agaaagagaa aaagcagaaa
      301 gtagatgaag atgagattca gaagatgcaa atcctggttt cttctttttc tgaggagcag
      361 ctgaaccgtt atgaaatgta tcgccgctca gctttcccta aggcagccat caaaaggcac
      421 tggatgtgtg tgagaagtgg ggagaaatgc caccactaca acccaaacat atagtaatga
      481 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      541 gacccggtgg tgagcgtaat cgcctg
//
