LOCUS       sequence-27850-e         764 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, 
            Kaelin WG
  TITLE     Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor
            suppressor protein requires binding to complexes containing elongins
            B/C and Cul2.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):732-41.
  PUBMED    9447969
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Feb . 18(2):732-41."
COMMENT     Sequence Label: HA-VHL 1-187-pRc/CMV
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(572..598)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(578..604)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(626..645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(627..645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(690..741)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(719..739)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cttcgtagag cgacctgacg atgtccagtc tcctgtaatt ctcaggcttg actaggctcc
       61 ggacaacctg gaggcatcgc tctttcagag tatacactgg cagtgtgata ttggcaaaaa
      121 taggctgtcc gtcaacattg agagatggca caaataattc agtttggtta accagaagcc
      181 catcgtgtgt ccctgcatct ctgaagagcc aaaggtgacc tcggtagctg tggatgcggc
      241 ggcccgtgcc aggcggcagc gttgggtagg gctgcggctc gccgtcgaag ttgagccata
      301 cgggcagcac gacgcgcgga ctgcgattgc agaagatgac ctgggagggc tcgcgcgagt
      361 tcaccgagcg cagcacgggc cgcggccgcc cggcctccat ctcctcctcg gcgcccagtt
      421 cctccgggcc ggactcttcc gggccggact cctcggcgcc cgactcctcc ccgccgtctt
      481 cttcagggcc gtactcttcg acgcctgcct cctccgcgcc tacctcggcc tcgtcccagt
      541 tctccgccct ccggggcatg gtggcggatc cgagggaggc gtagtcgggc acgtcgtang
      601 ggtaggccat ggtaagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag
      661 taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca
      721 cgcctaccgc ccatttgcgt caatggggcg ganttgttac naca
//
