LOCUS       sequence-278506-        1721 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2613
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtcactg tcgcctctgc cacgggaagt tttcctcgag aagcctgcgc
       61 agcatctccg agagggcgcc tggagcgagc atggagaggc catccgcaga ggagcgcgtg
      121 ctcgtacggg acttccagcg cctgcttggt gtggctgtcc gccaggaccc caccttgtct
      181 ccgtttgtct gcaagagctg ccacgcccag ttctaccagt gccacagcct tctcaagtcc
      241 ttcctgcaga gggtcaacgc ctccccggct ggtcgccgga agccttgtgc aaaggtcggt
      301 gcccagcccc caacaggggc agaggaggga gcgtgtctgg tggatctgat cacatccagc
      361 ccccagtgcc tgcacggctt ggtggggtgg gtgcatggac atgcggccag ctgcggggcc
      421 ctgccccacc ttcagaggac actgtcctcc gagtactgcg gcgtcatcca ggtcgtgtgg
      481 ggctgcgacc agggccacga ctacaccatg gataccagct ccagctgcaa ggccttcttg
      541 ctggacagtg cgctggcagt caagtggcca tgggacaaag agacggcgcc acggctgccc
      601 cagcaccgag ggtggaaccc tggggatgcc cctcagacct cccagggtag agggacaggg
      661 accccagttg gggctgagac caagaccctg cccagcacgg atgtggccca gcctccttcg
      721 gacagcgacg cggtggggcc caggtcgggc ttcccacctc agccaagcct gcccctttgc
      781 agggccccag ggcagttggg tgagaagcag cttccatctt caacctcgga tgatcgggta
      841 aaagacgagt tcagtgacct ttctgaggga gacgtcttga gtgaagatga aaatgacaag
      901 aagcaaaatg cccagtcttc ggacgagtcc tttgagcctt acccagaaag gaaagtctct
      961 ggtaagaaga gtgaaagcaa agaagccaag aagtctgaag aaccaagaat tcggaagaag
     1021 ccgggaccca agcccggatg gaagaagaag cttcgttgtg agagggagga gcttcccacc
     1081 atctacaagt gtccttacca gggctgcacg gccgtgtacc gaggcgctga cggcatgaag
     1141 aagcacatca aggagcacca cgaggaggtc cgggagcggc cctgccccca ccctggctgc
     1201 aacaaggttt tcatgatcga ccgctacctg cagcgccacg tgaagctcat ccacacagag
     1261 gtgcggaact atatctgtga cgaatgtgga caaaccttca agcagcggaa gcaccttctc
     1321 gtccaccaaa tgcgacattc gggagccaag cctttgcagt gtgaggtctg tgggttccag
     1381 tgcaggcagc gggcatccct caagtaccac atgaccaaac acaaggctga gactgagctg
     1441 gactttgcct gtgaccagtg tggccggcgg tttgagaagg cccacaacct caatgtacac
     1501 atgtccatgg tgcacccgct gacacagacc caggacaagg ccctgcccct ggaggcggaa
     1561 ccaccacctg ggccaccgag cccctctgtg accacagagg gccaggcggt gaagcccgaa
     1621 cccacctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1681 tcttgtggaa aggacgaggg agctctcgaa tggatctccc g
//
