LOCUS       sequence-279452-        2186 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2664
FEATURES             Location/Qualifiers
     source          1..2186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaataa aagcctggag agctcccaga cagacctgaa gctggtggcc
       61 cacccccgcg ccaagagcaa ggtgtggaag tatttcggct tcgacaccaa cgccgaggga
      121 tgcatcctgc agtggaagaa aatctactgc cgcatctgca tggcccagat cgcctactcc
      181 ggaaacacct ccaacctgtc ctaccacctg gagaagaacc accccgagga attctgcgag
      241 ttcgtcaaga gcaacacgga gcagatgcgt gaagccttcg ccaccgcctt ctccaagctg
      301 aagcccgagt cgtcccagca gcccgggcag gacgcgctgg ccgtcaaggc cggccacggc
      361 tacgacagca agaagcagca ggagctgacg gccgccgtgc tgggcctcat ctgcgagggg
      421 ctgtacccag cctccatcgt ggacgagccc accttcaagg tgctgctgaa gacggccgac
      481 ccccggtatg agctgcccag ccggaagtac atctctacca aggccatccc tgagaagtac
      541 ggggccgtcc gggaggtgat cctgaaggag ctggccgagg ccacctggtg tggcatctcc
      601 accgacatgt ggaggagtga gaatcagaac cgcgcctacg tcacgctggc cgcccacttc
      661 ctgggcctgg gcgcccccaa ctgcctgtcc atgggctccc gctgcctgaa gaccttcgag
      721 gtgcccgaag agaacacggc ggagaccatc acgcgagtgc tctatgaggt cttcatcgag
      781 tggggcatca gcgccaaggt cttcggggcc accaccaact atggcaagga catcgtgaag
      841 gcgtgctccc tgctggacgt cgcagtgcac atgccctgcc tgggccacac cttcaatgcc
      901 ggcatccagc aggccttcca gctcccgaag ctgggggcgc tgctgtcgcg ctgccgcaaa
      961 ctggtggagt acttccagca gtctgccgtg gccatgtaca tgctctatga gaagcagaag
     1021 cagcagaacg tggcccactg catgctggtg agcaaccgcg tctcctggtg ggggagcacg
     1081 ctggccatgc tgcagcgcct caaggagcag cagttcgtca tcgccggggt cttggtggag
     1141 gacagcaaca accaccacct catgctggag gccagcgagt gggccaccat cgaggggctg
     1201 gtggagctcc tgcagccctt caagcaggtg gccgagatgc tgtcggcctc caggtacccc
     1261 accatcagca tggtgaagcc gctgctgcac atgctcctga acaccacgct caacatcaag
     1321 gagaccgact ccaaggagct cagcatggcc aaggaggtca tcgccaagga gctttccaag
     1381 acctaccagg agacgcccga gatcgacatg tttctcaacg tggccacctt cctggacccc
     1441 cgctacaaga ggctgccctt cctctccgcc ttcgagcggc agcaggtgga gaatcgcgtg
     1501 gtggaagagg ccaagggcct gctggacaag gtcaaagacg gcggctaccg gccggctgag
     1561 gacaagatct tcccggtgcc cgaggagcct cccgtcaaga agctcatgcg gacatccacg
     1621 ccgccgcccg ccagcgtcat caacaacatg ctggccgaga tcttctgcca gacaggcggc
     1681 gtggaggacc aggaagagtg gcatgcccag gtggtggagg agctgagcaa cttcaagtcc
     1741 cagaaggtgc ttggcctcaa cgaagacccc ctcaagtggt ggtcagaccg cctggccctc
     1801 ttccccctgc tgcccaaggt gctgcagaag tactggtgcg tgacggccac gcgcgtcgcc
     1861 cctgagcgtc tcttcggatc cgccgccaac gtggtcagcg ccaagaggaa ccggctggct
     1921 cccgcgcacg tggacgagca ggtgtttctg tatgagaacg cccggagtgg ggcagaggcg
     1981 gaacccgagg accaggacga gggggagtgg ggcctggacc aggagcaggt gttctccttg
     2041 ggggatggcg tcagcggcgg tttctttggc attagggaca gcagcttcct gtagtaatga
     2101 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2161 gaaaatctaa agaaagctaa tggtct
//
