LOCUS       sequence-279518-         683 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0098
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             548..556
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggacaacct gcgcgagact ttcctcagcc tcgaggatgg cttgggctcc
       61 tctgacagcc ctggcctgct gtcttcctgg gactggaagg acagggcagg gccctttgag
      121 ctgaatcagg cctccccctc tcagagcctt tccccggctc catcgctgga atcctattct
      181 tcttctccct gtccagctgt ggctgggctg ccctgtgagc acggcggggc cagcagtggg
      241 ggcagcgaag gctgcagtgt cggtggggcc agtggcctgg tagaggtgga ctacaatatg
      301 ttagctttcc agcccaccca ccttcagggc ggtggtggcc ccaaggccca gaagggcacc
      361 aaagtcagga tgtctgtcca gcggaggcgg aaagccagcg agagggagaa gctcaggatg
      421 aggaccttgg cagatgccct gcacaccctc cggaattacc tgccacctgt ctacagccag
      481 agaggccagc ctctcaccaa gatccagaca ctcaagtaca ccatcaagta catcggggaa
      541 ctcacagacc tccttaaccg cggcagagag cccagagccc agagcgcgta gtaatgaact
      601 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      661 tcccagaaac agcccactgc gta
//
