LOCUS       sequence-279539-        2087 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0156
FEATURES             Location/Qualifiers
     source          1..2087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc
       61 tacagtgctt acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg
      121 caacaaagct atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc
      181 actgcaacct atgggcagac cgcctatgca acttcttatg gacagcctcc cactgtagaa
      241 gggaccagta caggttatac tactccaact gccccccagg catacagcca gcctgtccag
      301 gggtatggca ctggtgctta tgataccacc actgctacag tcaccaccac ccaggcctcc
      361 tatgcagctc agtctgcata tggcactcag cctgcttatc cagcctatgg gcagcagcca
      421 gcagccactg cacctacaag accgcaggat ggaaacaagc ccactgagac tagtcaacct
      481 caatctagca cagggggtta caaccagccc agcctaggat atggacagag taactacagt
      541 tatccccagg tacctgggag ctaccccatg cagccagtca ctgcacctcc atcctaccct
      601 cctaccagct attcctctac acagccgact agttatgatc agagcagtta ctctcagcag
      661 aacacctatg ggcaaccgag cagctatgga cagcagagta gctatggtca acaaagcagc
      721 tatgggcagc agcctcccac tagttaccca ccccaaactg gatcctacag ccaagctcca
      781 agtcaatata gccaacagag cagcagctac gggcagcaga gttcattccg acaggaccac
      841 cccagtagca tgggtgttta tgggcaggag tctggaggat tttccggacc aggagagaac
      901 cggagcatga gtggccctga taaccggggc aggggaagag ggggatttga tcgtggaggc
      961 atgagcagag gtgggcgggg aggaggacgc ggtggaatgg gcgctggaga gcgaggtggc
     1021 ttcaataagc ctggtggacc catggatgaa ggaccagatc ttgatctagg cccacctgta
     1081 gatccagatg aagactctga caacagtgca atttatgtac aaggattaaa tgacagtgtg
     1141 actctagatg atctggcaga cttctttaag cagtgtgggg ttgttaagat gaacaagaga
     1201 actgggcaac ccatgatcca catctacctg gacaaggaaa caggaaagcc caaaggcgat
     1261 gccacagtgt cctatgaaga cccacccact gccaaggctg ccgtggaatg gtttgatggg
     1321 aaagattttc aagggagcaa acttaaagtc tcccttgctc ggaagaagcc tccaatgaac
     1381 agtatgcggg gtggtctgcc accccgtgag ggcagaggca tgccaccacc actccgtgga
     1441 ggtccaggag gcccaggagg tcctggggga cccatgggtc gcatgggagg ccgtggagga
     1501 gatagaggag gcttccctcc aagaggaccc cggggttccc gagggaaccc ctctggagga
     1561 ggaaacgtcc agcaccgagc tggagactgg cagtgtccca atccgggttg tggaaaccag
     1621 aacttcgcct ggagaacaga gtgcaaccag tgtaaggccc caaagcctga aggcttcctc
     1681 ccgccaccct ttccgccccc gggtggtgat cgtggcagag gtggccctgg tggcatgcgg
     1741 ggaggaagag gtggcctcat ggatcgtggt ggtcccggtg gaatgttcag aggtggccgt
     1801 ggtggagaca gaggtggctt ccgtggtggc cggggcatgg accgaggtgg ctttggtgga
     1861 ggaagacgag gtggccctgg ggggccccct ggacctttga tggaacagat gggaggaaga
     1921 agaggaggac gtggaggacc tggaaaaatg gataaaggcg agcaccgtca ggagcgcaga
     1981 gatcggccct actagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2041 tatatatctt gtggaaagga cgaagaggtg aatactttga tcattcc
//
