LOCUS       sequence-279542-        1904 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0157
FEATURES             Location/Qualifiers
     source          1..1904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc
       61 tacagtgctt acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg
      121 caacaaagct atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc
      181 actgcaacct atgggcagac cgcctatgca acttcttatg gacagcctcc cactggttat
      241 actactccaa ctgcccccca ggcatacagc cagcctgtcc aggggtatgg cactggtgct
      301 tatgatacca ccactgctac agtcaccacc acccaggcct cctatgcagc tcagtctgca
      361 tatggcactc agcctgctta tccagcctat gggcagcagc cagcagccac tgcacctaca
      421 agctattcct ctacacagcc gactagttat gatcagagca gttactctca gcagaacacc
      481 tatgggcaac cgagcagcta tggacagcag agtagctatg gtcaacaaag cagctatggg
      541 cagcagcctc ccactagtta cccaccccaa actggatcct acagccaagc tccaagtcaa
      601 tatagccaac agagcagcag ctacgggcag cagagttcat tccgacagga ccaccccagt
      661 agcatgggtg tttatgggca ggagtctgga ggattttccg gaccaggaga gaaccggagc
      721 atgagtggcc ctgataaccg gggcagggga agagggggat ttgatcgtgg aggcatgagc
      781 agaggtgggc ggggaggagg acgcggtgga atgggcagcg ctggagagcg aggtggcttc
      841 aataagcctg gtggacccat ggatgaagga ccagatcttg atctaggccc acctgtagat
      901 ccagatgaag actctgacaa cagtgcaatt tatgtacaag gattaaatga cagtgtgact
      961 ctagatgatc tggcagactt ctttaagcag tgtggggttg ttaagatgaa caagagaact
     1021 gggcaaccca tgatccacat ctacctggac aaggaaacag gaaagcccaa aggcgatgcc
     1081 acagtgtcct atgaagaccc acccactgcc aaggctgccg tggaatggtt tgatgggaaa
     1141 gattttcaag ggagcaaact taaagtctcc cttgctcgga agaagcctcc aatgaacagt
     1201 atgcggggtg gtctgccacc ccgtgagggc agaggcatgc caccaccact ccgtggaggt
     1261 ccaggaggcc caggaggtcc tgggggaccc atgggtcgca tgggaggccg tggaggagat
     1321 agaggaggct tccctccaag aggaccccgg ggttcccgag ggaacccctc tggaggagga
     1381 aacgtccagc accgagctgg agactggcag tgtcccaatc cgggttgtgg aaaccagaac
     1441 ttcgcctgga gaacagagtg caaccagtgt aaggccccaa agcctgaagg cttcctcccg
     1501 ccaccctttc cgcccccggg tggtgatcgt ggcagaggtg gccctggtgg catgcgggga
     1561 ggaagaggtg gcctcatgga tcgtggtggt cccggtggaa tgttcagagg tggccgtggt
     1621 ggagacagag gtggcttccg tggtggccgg ggcatggacc gaggtggctt tggtggagga
     1681 agacgaggtg gccctggggg gccccctgga cctttgatgg aacagatggg aggaagaaga
     1741 ggaggacgtg gaggacctgg aaaaatggat aaaggcgagc accgtcagga gcgcagagat
     1801 cggccctact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1861 atatcttgtg gaaaggacga tttaacattt cctggatcat taag
//
