LOCUS       sequence-279569-         674 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0184
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggacga aggccagacc aaaatcaagc agaggcgaag tcggaccaat
       61 ttcaccctgg aacaactcaa tgagctggag aggctttttg acgagaccca ctatcccgac
      121 gccttcatgc gagaggaact gagccagcga ctgggcctgt cggaggcccg agtgcaggtt
      181 tggtttcaaa atcgaagagc taaatgtaga aaacaagaaa atcaactcca taaaggtgtt
      241 ctcatagggg ccgccagcca gtttgaagct tgtagagtcg caccttatgt caacgtaggt
      301 gctttaagga tgccatttca gcaggttcag gcgcagctgc agctggacag cgctgtggcg
      361 cacgcgcacc accacctgca tccgcacctg gccgcgcacg cgccctacat gatgttccca
      421 gcaccgccct tcggactgcc gctcgccacg ctggccgcgg attcggcttc cgccgcctcg
      481 gtagtggcgg ccgcagcagc cgccaagacc accagcaaga actccagcat cgccgatctc
      541 agactgaaag ccaaaaagca cgccgcagcc ctgggtctgt agtaatgaac tagtccgtaa
      601 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gggcccggat
      661 ccgcatgcgc tatt
//
