LOCUS       sequence-279668-        1355 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0286
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggagaaa aaagattcag attacgagga ttatggatga acgtaacaga
       61 caggtgacat ttacaaagag gaaatttggg ttgatgaaga aggcttatga gctgagcgtg
      121 ctgtgtgact gtgagattgc gctgatcatc ttcaacagca ccaacaagct gttccagtat
      181 gccagcaccg acatggacaa agtgcttctc aagtacacgg agtacaacga gccgcatgag
      241 agccggacaa actcagacat cgtggaggct gttccacctc ccaacttcga gatgccagtc
      301 tccatcccag tgtccagcca caacagtttg gtgtacagca accctgtcag ctcactggga
      361 aaccccaacc tattgccact ggctcaccct tctctgcaga ggaatagtat gtctcctggt
      421 gtaacacatc gacctccaag tgcaggtaac acaggtggtc tgatgggtgg agacctcacg
      481 tctggtgcag gcaccagtgc agggaacggg tatggcaatc cccgaaactc accaggtctg
      541 ctggtctcac ctggtaactt gaacaagaat atgcaagcaa aatctcctcc cccaatgaat
      601 ttaggaatga ataaccgtaa accagatctc cgagttctta ttccaccagg cagcaagaat
      661 acgatgccat cagtgaatca aaggataaat aactcccagt cggctcagtc attggctacc
      721 ccagtggttt ccgtagcaac tcctacttta ccaggacaag gaatgggagg atatccatca
      781 gccatttcaa caacatatgg taccgagtac tctctgagta gtgcagacct gtcatctctg
      841 tctgggttta acaccgccag cgctcttcac cttggttcag taactggctg gcaacagcaa
      901 cacctacata acatgccacc atctgccctc agtcagttgg gagcttgcac tagcactcat
      961 ttatctcaga gttcaaatct ctccctgcct tctactcaaa gcctcaacat caagtcagaa
     1021 cctgtttctc ctcctagaga ccgtaccacc accccttcga gatacccaca acacacgcgc
     1081 cacgaggcgg ggagatctcc tgttgacagc ttgagcagct gtagcagttc gtacgacggg
     1141 agcgaccgag aggatcaccg gaacgaattc cactccccca ttggactcac cagaccttcg
     1201 ccggacgaaa gggaaagtcc ctcagtcaag cgcatgcgac tttctgaagg atgggcaaca
     1261 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1321 ggaaaggacg acgtatagct ctaggtcact tcgtt
//
