LOCUS       sequence-279707-        1613 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2523
FEATURES             Location/Qualifiers
     source          1..1613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggaaca cggagtgacc caaaccgaac atatggctac catagaagca
       61 catgcagtgg cccagcaagt gcagcaggtc catgtggcta cttacaccga gcatagtatg
      121 ctgagtgctg atgaagactc gccttcttct cccgaggaca cctcttacga tgactcagat
      181 atactcaact ccacagcagc tgatgaggtg acagctcatc tggcagctgc aggtcctgtg
      241 ggaatggccg ctgctgctgc tgtggcaaca ggaaagaaac ggaaacggcc tcatgtattt
      301 gagtctaatc catctatccg gaagaggcaa caaacacgtt tgcttcggaa acttcgagcc
      361 acgttagatg aatatactac tcgtgtggga cagcaagcta ttgtcctctg tatctcaccc
      421 tccaaaccta accctgtctt taaagtgttt ggtgcagcac ctttggagaa tgtggtgcgt
      481 aagtacaaga gcatgatcct ggaagacctg gagtctgctc tggcagaaca cgcccctgcg
      541 ccacaggagg ttaactcaga actgccgcct ctcaccatcg acggaattcc agtctctgtg
      601 gacaaaatga cccaggccca gcttcgggca tttatcccag agatgctcaa gtactctaca
      661 ggtcggggaa aaccaggctg ggggaaagaa agctgcaagc ccatctggtg gcctgaagat
      721 atcccctggg caaatgtccg gagtgatgtc cgcacagaag agcaaaagca gagggtttca
      781 tggacccagg cactacggac catagttaaa aactgttata aacagcatgg gcgggaagac
      841 cttttgtatg cctttgaaga tcagcaaacg caaacacagg ccacagccac acatagtata
      901 gctcatcttg taccatcaca gactgtagtc cagactttta gtaaccctga tggcactgtc
      961 tcacttatcc aggttggtac gggggcaaca gtagccacat tggctgatgc ttcagaattg
     1021 ccaaccacgg tcaccgttgc ccaagtgaat tattctgccg tggctgatgg agaggtggaa
     1081 caaaattggg ccacgttaca gggaggtgag atgaccatcc agacgacgca agcatcagag
     1141 gccacccagg cggtggcatc gttggcagag gccgcagtgg cagcttctca ggagatgcag
     1201 cagggagcta cagtcactat ggcgcttaac agcgaagctg ccgcccatgc tgtcgccacc
     1261 ctggctgagg ccaccttaca aggtggggga cagatcgtct tgtctgggga aaccgcagca
     1321 gccgtcggag cacttactgg agtccaagat gctaatggcc tggtccagat ccctgtgagc
     1381 atgtaccaga ctgtggtgac cagcctcgcc cagggcaacg gaccagtgca ggtggccatg
     1441 gcccctgtga ccaccaggat atcagacagc gcagtcacca tggacggcca agctgtggag
     1501 gtggtgacat tggaacagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1561 tggctttata tatcttgtgg aaaggacgat tgcgtccgga ctgcagcttg acg
//
