LOCUS       sequence-279824-        2183 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0218
FEATURES             Location/Qualifiers
     source          1..2183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             808..816
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgatgcaaga atctgggact gagacaaaaa gtaacggttc agccatccag
       61 aatgggtcgg gcggcagcaa ccacttacta gagtgcggcg gtcttcggga ggggcggtcc
      121 aacggagaga cgccggccgt ggacatcggg gcagctgacc tcgcccacgc ccagcagcag
      181 cagcaacagg cacttcaggt ggcaagacag ctccttcttc agcagcaaca gcagcagcaa
      241 gttagtggat taaaatctcc caagaggaat gacaaacaac cagctcttca ggttcccgtg
      301 tcagtggcta tgatgacacc tcaagttatc actccccagc aaatgcagca gatcctccag
      361 caacaagtgc tgagccctca gcagctccag gttctcctcc agcagcagca ggccctcatg
      421 cttcaacagc agcagcttca agagttttat aaaaaacaac aggaacagtt gcagcttcaa
      481 cttttacaac aacaacatgc tggaaaacag cctaaagagc aacagcaggt ggctacccag
      541 cagttggctt ttcagcagca gcttttacag atgcagcagt tacagcagca gcacctcctg
      601 tctttgcagc gccaaggcct tctgacaatt cagcccgggc agcctgccct tccccttcaa
      661 cctcttgctc aaggcatgat tccaacagaa ctgcagcagc tctggaaaga agtgacaagt
      721 gctcatactg cagaagaaac cacaggcaac aatcacagca gtttggatct gaccacgaca
      781 tgtgtctcct cctctgcacc ttccaagacc tccttaataa tgaacccaca tgcctctacc
      841 aatggacagc tctcagtcca cactcccaaa agggaaagtt tgtcccatga ggagcacccc
      901 catagccatc ctctctatgg acatggtgta tgcaagtggc caggctgtga agcagtgtgc
      961 gaagatttcc aatcatttct aaaacatctc aacagtgagc atgcgctgga cgatagaagt
     1021 acagcccaat gtagagtaca aatgcaggtt gtacagcagt tagagctaca gcttgcaaaa
     1081 gacaaagaac gcctgcaagc catgatgacc cacctgcatg tgaagtctac agaacccaaa
     1141 gccgcccctc agcccttgaa tctggtatca agtgtcactc tctccaagtc cgcatcggag
     1201 gcttctccac agagcttacc tcatactcca acgaccccaa ccgcccccct gactcccgtc
     1261 acccaaggcc cctctgtcat cacaaccacc agcatgcaca cggtgggacc catccgcagg
     1321 cggtactcag acaaatacaa cgtgcccatt tcgtcagcag atattgcgca gaaccaagaa
     1381 ttttataaga acgcagaagt tagaccacca tttacatatg catctttaat taggcaggcc
     1441 attctcgaat ctccagaaaa gcagctaaca ctaaatgaga tctataactg gttcacacga
     1501 atgtttgctt acttccgacg caacgcggcc acgtggaagg gtgccattcg caccaacctc
     1561 tcactgcata agtgctttat cagagtagag gatgagtttg ggtccttttg gacagttgat
     1621 gatgaagagt ttaaacgtgg ccgccatatt caacgaggcc gtcctcgcaa atactgcccc
     1681 gatgaaaact ttgacgagct tgtcgcacat aacccttccc ttattaaaaa catgcagagc
     1741 agccacgcct actgcacacc tctcaatgca gctttacagg cttcaatggc tgagaatagt
     1801 atacctctat acactaccgc ttccatggga aatcccactc tgggcaactt agccagcgca
     1861 atacgggaag agctgaacgg ggcaatggag cataccaaca gcaacgagag tgacagcagt
     1921 ccaggcagat ctcctatgca agccgtgcat cctgtacacg tcaaagaaga gcccctcgat
     1981 ccagaggaag ctgaagggcc cctgtcctta gtgacaacag ccaaccacag tccagatttt
     2041 gaccatgaca gagattacga agatgaacca gtaaacgagg acatggagta gtaatgaact
     2101 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     2161 atcctggcgg aaattccctc ttt
//
