LOCUS       sequence-279899-        1553 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0291
FEATURES             Location/Qualifiers
     source          1..1553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggagaaa aaagattcag attacgagga ttatggatga acgtaacaga
       61 caggtgacat ttacaaagag gaaatttggg ttgatgaaga aggcttatga gctgagcgtg
      121 ctgtgtgact gtgagattgc gctgatcatc ttcaacagca ccaacaagct gttccagtat
      181 gccagcaccg acatggacaa agtgcttctc aagtacacgg agtacaacga gccgcatgag
      241 agccggacaa actcagacat cgtggagacg ttgagaaaga agggccttaa tggctgtgac
      301 agcccagacc ccgatgcgga cgattccgca ttgaacaaga aagaaaacaa aggctgtgaa
      361 agccccgatc ccgactcctc ttatgcactc accccacgca ctgaagaaaa atacaaaaaa
      421 attaatgaag aatttgataa tatgatcaag agtcataaaa ttcctgctgt tccacctccc
      481 aacttcgaga tgccagtctc catcccagtg tccagccaca acagtttggt gtacagcaac
      541 cctgtcagct cactgggaaa ccccaaccta ttgccactgg ctcacccttc tctgcagagg
      601 aatagtatgt ctcctggtgt aacacatcga cctccaagtg caggtaacac aggtggtctg
      661 atgggtggag acctcacgtc tggtgcaggc accagtgcag ggaacgggta tggcaatccc
      721 cgaaactcac caggtctgct ggtctcacct ggtaacttga acaagaatat gcaagcaaaa
      781 tctcctcccc caatgaattt aggaatgaat aaccgtaaac cagatctccg agttcttatt
      841 ccaccaggca gcaagaatac gatgccatca gtgaatcaaa ggataaataa ctcccagtcg
      901 gctcagtcat tggctacccc agtggtttcc gtagcaactc ctactttacc aggacaagga
      961 atgggaggat atccatcagc catttcaaca acatatggta ccgagtactc tctgagtagt
     1021 gcagacctgt catctctgtc tgggtttaac accgccagcg ctcttcacct tggttcagta
     1081 actggctggc aacagcaaca cctacataac atgccaccat ctgccctcag tcagttggga
     1141 gcttgcacta gcactcattt atctcagagt tcaaatctct ccctgccttc tactcaaagc
     1201 ctcaacatca agtcagaacc tgtttctcct cctagagacc gtaccaccac cccttcgaga
     1261 tacccacaac acacgcgcca cgaggcgggg agatctcctg ttgacagctt gagcagctgt
     1321 agcagttcgt acgacgggag cgaccgagag gatcaccgga acgaattcca ctcccccatt
     1381 ggactcacca gaccttcgcc ggacgaaagg gaaagtccct cagtcaagcg catgcgactt
     1441 tctgaaggat gggcaacata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1501 tggctttata tatcttgtgg aaaggacgag ccgtacgcaa tcccttccta gtt
//
