LOCUS       sequence-280085-         521 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0571
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggaccag cagcctgggc ctggtggaca ccacaggagg cccaggcgat
       61 gactacgggg tgcttgggag cactgccaat gagacagaga agaaatcatc caggcggaga
      121 aaggagagtt cagacaacca ggagaacaga gggaagccgg agggcagcag caaagcccgc
      181 aaggagagga cggccttcac caaggagcag ctgcgagagc tggaggcaga gtttgcccat
      241 cataactacc tgactcggct ccgcagatat gagattgcgg taaacctgga cctctctgag
      301 cgccaggtca aagtgtggtt ccagaaccga aggatgaagt ggaagcgtgt gaagggaggt
      361 cagcccatct cccccaatgg gcaggaccct gaggatgggg actccacagc ctctccaagt
      421 tcagagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
      481 tcttgtggaa aggacgagtt acataccctc gactattatc g
//
