LOCUS       sequence-280376-        1445 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1182
FEATURES             Location/Qualifiers
     source          1..1445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttcac ggcgtccccc aagccccaac tctcctctcg ggccaacgcc
       61 ttctccattg ccgcgctcat gtcgagcggc ggctctaagg agaaggaggc gacggagaac
      121 acaatcaaac ccctggagca atttgtggag aagtcgtcct gtgcccagcc cctgggtgag
      181 ctgaccagcc tggatgctca tggggagttt ggtggaggca gtggcagcag cccgtcctcc
      241 tcctctctgt gcactgagcc actgatcccc accaccccca tcatccccag tgaggaaatg
      301 gccaaaattg cctgcagcct ggagaccaag gagctttggg acaaattcca tgagctgggc
      361 accgagatga tcatcaccaa gtcgggcagg aggatgtttc caaccatccg ggtgtccttt
      421 tcgggggtgg atcctgaggc caagtacata gtcctgatgg acatcgtccc tgtggacaac
      481 aagaggtacc gctacgccta ccaccggtcc tcctggctgg tggctggcaa ggccgacccg
      541 ccgttgccag ccaggctcta tgtgcatcca gattctcctt ttaccggtga gcaactactc
      601 aaacagatgg tgtcttttga aaaggtgaaa ctcaccaaca atgaactgga tcaacatggc
      661 catataattt tgaactcaat gcataagtac cagccaaggg tgcacatcat taagaagaaa
      721 gaccacacag cctcattgct caacctgaag tctgaagaat ttagaacttt catctttcca
      781 gaaacagttt ttacggcagt cactgcctac cagaatcaac tgataacgaa gctgaaaata
      841 gatagcaatc cttttgccaa aggattccgg gattcctcca ggctcactga cattgagagg
      901 gaaagtgtgg agagcctgat tcaaaagcat tcctatgcac gctcacccat ccgtacctac
      961 ggaggagaag aagatgtctt gggggatgag agtcagacaa ccccaaatcg agggtcagcc
     1021 tttacaacat ctgataattt gtctctcagc tcctgggtat catcttcttc cagttttcct
     1081 gggtttcagc acccacagtc cctgactgct cttggcacca gcacagcatc catagcaaca
     1141 cccattcctc accccatcca gggttctctg ccaccatata gccgactggg aatgcctctg
     1201 acaccatcgg ccattgccag ctccatgcaa gggagtggcc ccacattccc ttcattccac
     1261 atgccgcgat accatcacta ttttcagcag gggccctatg ctgccattca aggactacgc
     1321 cattcctctg ctgtgatgac gccatttgta tagtaatgaa ctagtccgta acttgaaagt
     1381 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggttgtgtg attgccagga
     1441 caatt
//
