LOCUS       sequence-280583-        1961 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1649
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctccgg tgcgtataac ccgtatatag agataattga acaacccagg
       61 cagaggggaa tgcgttttag atacaaatgt gaagggcgat cagcaggcag cattccaggg
      121 gagcacagca cagacaacaa ccgaacatac ccttctatcc agattatgaa ctattatgga
      181 aaaggaaaag tgagaattac attagtaaca aagaatgacc catataaacc tcatcctcat
      241 gatttagttg gaaaagactg cagagacggc tactatgaag cagaatttgg acaagaacgc
      301 agacctttgt ttttccaaaa tttgggtatt cgatgtgtga agaaaaaaga agtaaaagaa
      361 gctattatta caagaataaa ggcaggaatc aatccattca atgtccctga aaaacagctg
      421 aatgatattg aagattgtga cctcaatgtg gtgagactgt gttttcaagt ttttctccct
      481 gatgaacatg gtaatttgac gactgctctt cctcctgttg tctcgaaccc aatttatgac
      541 aaccgtgctc caaatactgc agaattaagg atttgtcgtg taaacaagaa ttgtggaagt
      601 gtcagaggag gagatgaaat atttctactt tgtgacaaag ttcagaaaga tgacatagaa
      661 gttcgttttg tgttgaacga ttgggaagca aaaggcatct tttcacaagc tgatgtacac
      721 cgtcaagtag ccattgtttt caaaactcca ccatattgca aagctatcac agaacccgta
      781 acagtaaaaa tgcagttgcg gagaccttct gaccaggaag ttagtgaatc tatggatttt
      841 agatatctgc cagatgaaaa agatacttac ggcaataaag caaagaaaca aaagacaact
      901 ctgcttttcc agaaactgtg ccaggatcac gtagaaacag ggtttcgcca tgttgaccag
      961 gatggtcttg aactcctgac atcaggtgat ccacccacct tggcctccca aagtgctggg
     1021 attacagtta attttcctga gagaccaaga cctggtctcc tcggttcaat tggagaagga
     1081 agatacttca aaaaagaacc aaacttgttt tctcatgatg cagttgtgag agaaatgcct
     1141 acaggggttt caagtcaagc agaatcctac tatccctcac ctgggcccat ctcaagtgga
     1201 ttgtcacatc atgcctcaat ggcacctctg ccttcttcaa gctggtcatc agtggcccac
     1261 cccaccccac gctcaggcaa tacaaaccca ctgagtagtt tttcaacaag gacacttcct
     1321 tctaattcgc aaggtatccc accattcctg agaatacctg ttgggaatga tttaaatgct
     1381 tctaatgctt gcatttacaa caatgccgat gacatagtcg gaatggaagc gtcatccatg
     1441 ccatcagcag atttatatgg tatttctgat cccaacatgc tgtctaattg ttctgtgaat
     1501 atgatgacaa ccagcagtga cagcatggga gagactgata atccaagact tctgagcatg
     1561 aatcttgaaa acccctcatg taattcagtg ttagacccaa gagacttgag acagctccat
     1621 cagatgtcct cttccagtat gtcagcaggc gccaattcca atactactgt ttttgtttca
     1681 caatcagatg catttgaggg atctgacttc agttgtgcag ataacagcat gataaatgag
     1741 tcgggaccat caaacagtac taatccaaac agtcatggtt ttgttcaaga tagtcagtat
     1801 tcaggtattg gcagtatgca aaatgagcaa ttgagtgact cctttccata tgaatttttt
     1861 caagtatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1921 tcttgtggaa aggacgaccc acattcatgt gaccgtccgt a
//
