LOCUS       sequence-280895-        2117 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2148
FEATURES             Location/Qualifiers
     source          1..2117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccac attccagctt ccagggcacc aggagatgcc gctgaccttc
       61 caggatgtgg ccgtgtactt ctctcaggcg gaggggcggc agctgggccc ccagcagcgg
      121 gcgctctacc gggatgtgat gctggagaac tatgggaacg tggcctctct gggattccct
      181 gtccctaagc cggagttgat ctcccagctg gagcagggga aggaactttg ggtcctgaat
      241 cttctgggag ctgaggaacc agatatcttg aaaagctgcc agaaagattc tgaggttggg
      301 accaagaagg aactatctat tttaaaccaa aaattttccg aagaagtaaa aaccccagaa
      361 tttgtatcaa gaagactctt aagggataat gcacaggccg ctgagtttcg ggaagcatgg
      421 ggccgtgagg gcaaactcaa agagcgcgtg ggaaattctg ccgggcagag tttgaacaaa
      481 cccaatattc acaagagagt tttaacagaa gctaccgtgg gcagggaaag atctttggga
      541 gaaagaaccc aagagtgtag tgcatttgat agaaacttga atctggacca aaatgttgtt
      601 agacttcaaa gaaataaaac aggagagagg gtctttaaat gtgatatatg cagcaaaacc
      661 ttcaaatata attcagacct aagtagacac cagagaagtc acactgggga gaagccgtac
      721 gaatgtggcc ggtgtgggcg agcctttact cacagctcaa atcttgttct gcaccatcac
      781 attcacactg gaaataaacc atttaaatgt gatgaatgtg ggaaaacttt tggactcaat
      841 tctcacctcc gtcttcatcg gagaattcac actggagaaa aaccctttgg ctgtggtgag
      901 tgtgggaagg ctttcagtcg aagctcaact cttattcaac atcggatcat tcacacagga
      961 gagaaaccct acaagtgtaa tgaatgtgga agaggcttta gccagagccc ccagttaact
     1021 cagcatcaga gaattcacac tggagagaag ccgcatgaat gcagtcactg tgggaaggcc
     1081 ttcagtcgaa gctccagcct tattcagcat gagagaattc acactggaga gaagccccat
     1141 aaatgcaatc agtgtgggaa ggccttcagt cagagctcaa gccttttcct ccatcatcgg
     1201 gttcatactg gagagaaacc ctatgtatgt aatgaatgcg gcagagcctt tggttttaac
     1261 tctcatctta ctgaacacgt aaggattcac acaggagaaa aaccctatgt ttgtaatgag
     1321 tgcggcaaag cctttcgtcg gagttccact cttgttcagc atcgaagagt tcacactggg
     1381 gagaagccct accagtgcgt tgaatgtggg aaagctttca gccagagctc ccagctcacc
     1441 ctacatcagc gagttcacac tggagagaag ccctatgact gtggtgactg tgggaaggcc
     1501 ttcagccgga ggtcaaccct cattcagcat cagaaagttc acagcggaga gactcgtaag
     1561 tgcagaaaac atggtccagc ctttgttcat ggctccagcc tcacagcaga tggacagatt
     1621 cccactggag agaagcacgg cagagccttt aaccatggtg caaatctcat tctgcgctgg
     1681 acagttcaca ctggtgagaa atcctttgga tgtaatgaat atggaaaagc tttcagtccc
     1741 acctcacgac ccactgaaga tcagataatg catgctgggg aaaagcccta taaatgtcaa
     1801 gaatgtggaa acgccttcag tggaaagtca acccttattc aacatcaggt aactcacact
     1861 ggtcagaaac catgtcattg cagtgtgtat gggaaagcct tcagccagag ttcacagctc
     1921 acaccacctc agcagactcg tgttggagag aaacctgctt taaatgatgg ctctaaaaga
     1981 tactttattc atatcaagaa gattttccaa gaaagacatt tttagtaatg aactagtccg
     2041 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaccgcagt
     2101 tgatggagga gtgcgga
//
