LOCUS       sequence-280976-        1505 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2465
FEATURES             Location/Qualifiers
     source          1..1505
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccctcca cccccgcaga gtccggctaa agccctggct ggtggcccag
       61 gtggatagtg gcctctaccc tgggctcatc tggctacaca gggactctaa acgcttccag
      121 attccctgga aacatgccac ccggcatagc cctcaacaag aagaggaaaa taccattttt
      181 aaggcctggg ctgtagagac agggaagtac caggaagggg tggatgaccc tgacccagct
      241 aaatggaagg cccagctgcg ctgtgctctc aataagagca gagaattcaa cctgatgtat
      301 gatggcacca aggaggtgcc catgaaccca gtgaagatat atcaagtgtg tgacatccct
      361 cagccccagg gctcgatcat taacccagga tccacagggt ctgctccctg ggatgagaag
      421 gataatgatg tggatgaaga agatgaggaa gatgagctgg atcagtcgca gcaccatgtt
      481 cccatccagg acaccttccc cttcctgaac atcaatggtt ctcccatggc gccagccagt
      541 gtgggcaatt gcagtgtggg caactgcagc ccggaggcag tgtggcccaa aactgaaccc
      601 ctggagatgg aagtacccca ggcacctata cagcccttct atagctctcc agaactgtgg
      661 atcagctctc tcccaatgac tgacctggac atcaagtttc agtaccgtgg gaaggagtac
      721 gggcagacca tgaccgtgag caaccctcag ggctgccgac tcttctatgg ggacctgggt
      781 cccatgcctg accaggagga gctctttggt cccgtcagcc tggagcaggt caaattccca
      841 ggtcctgagc atattaccaa tgagaagcag aagctgttca ctagcaagct gctggacgtc
      901 atggacagag gactgatcct ggaggtcagc ggtcatgcca tttatgccat caggctgtgc
      961 cagtgcaagg tgtactggtc tgggccatgt gccccatcac ttgttgctcc caacctgatt
     1021 gagagacaaa agaaggtcaa gctattttgt ctggaaacat tccttagcga tctcattgcc
     1081 caccagaaag gacagataga gaagcagcca ccgtttgaga tctacttatg ctttggggaa
     1141 gaatggccag atgggaaacc attggaaagg aaactcatct tggttcaggt cattccagta
     1201 gtggctcgga tgatctacga gatgttttct ggtgatttca cacgatcctt tgatagtggc
     1261 agtgtccgcc tgcagatctc aaccccagac atcaaggata acatcgttgc tcagctgaag
     1321 cagctgtacc gcatccttca aacccaggag agctggcagc ccatgcagcc cacccccagc
     1381 atgcaactgc cccctgccct gcctccccag tagtaatgaa ctagtccgta acttgaaagt
     1441 atttcgattt cttggcttta tatatcttgt ggaaaggacg acgttgtctg gagatgtgtg
     1501 tctga
//
