LOCUS       sequence-281063-        1748 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2687
FEATURES             Location/Qualifiers
     source          1..1748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacgttt aacaaaaaga cgacaggcgg atacaaaagc tatccagcat
       61 ctttgggcag ccattgagat tatacggaac cagaagcaga ttgccaacat tgaccgtatt
      121 acaaagtata tgtctcgagt ccacggtatg caccctaaag agaccacccg tcagctgagc
      181 ttagctgtga aagatggtct tattgtcgaa actctaacag tgggctgcaa aggttcaaaa
      241 gctggtattg aacaagaagg atattggttg ccaggagatg agattagcat taagaagaag
      301 aatacaaaca aacaggagat gggcacatac ctcagattca ttgtctcccg catgaaggag
      361 agggctatag atcttaataa aaaggggaag gacaataaac acccgatgta caggaggctg
      421 gtgcactcag ctgtggacgt tcccaccatt caagagaaag tgaatgaagg gaaataccga
      481 agttatgaag agttcaaagc tgatgcccaa ttgcttctcc acaataccgt gattttctat
      541 ggagcagaca gtgagcaagc tgacattgcg aggatgctat ataaagacac atgtcatgag
      601 ctggatgaac tgcagctttg caagaattgc ttttacttgt caaatgctcg tcctgacaac
      661 tggttctgtt atccttgtat acctaatcat gagctggttt gggctaaaat gaaaggtttt
      721 gggttttggc cagccaaagt catgcagaaa gaagacaatc aagtcgacgt tcgcttcttt
      781 ggccaccacc accagagggc ctggattcct tctgaaaaca ttcaagatat cacagtcaac
      841 attcatcggc tgcacgtgaa gcgcagtatg ggttggaaaa aggcctgtga tgagctggag
      901 ctgcatcagc gtttcctacg agaagggaga ttttggaaat ctaagaatga ggaccgaggt
      961 gaggaagagg cagaatccag tatctcctcc accagtaatg agcagctaaa ggtcactcaa
     1021 gaaccaagag caaagaaagg acgacgtaat caaagtgtgg agcccaaaaa ggaagaacca
     1081 gagcctgaaa cagaagcagt aagttctagc caggaaatac ccacgatgcc tcagcccatc
     1141 gaaaaagtct ccgtgtcaac tcagacaaag aagttaagtg cctcttcacc aagaatgctg
     1201 catcggagca cccagaccac aaacgacggc gtgtgtcaga gcatgtgcca tgacaaatac
     1261 accaagatct tcaatgactt caaagaccgg atgaagtcgg accacaagcg ggagacagag
     1321 cgtgttgtcc gagaagctct ggagaagctg cgttctgaaa tggaagaaga aaagagacaa
     1381 gctgtaaata aagctgtagc caacatgcag ggtgagatgg acagaaaatg taagcaagta
     1441 aaggaaaagt gtaaggaaga atttgtagaa gaaatcaaga agctggcaac acagcacaag
     1501 caactgattt ctcagaccaa gaagaagcag tggtgctaca actgtgagga ggaggccatg
     1561 taccactgct gctggaacac atcctactgc tccatcaagt gccagcagga gcactggcac
     1621 gcggagcaca agcgcacctg ccgccggaaa agatagtaat gaactagtcc gtaacttgaa
     1681 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaactgtc cggcttgcca
     1741 gtggatgg
//
