LOCUS       sequence-281102-        1976 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2781
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccataa atatgtgggt ttgcagtatc acggatcagt gacatttgag
       61 gatgtggcca tagccttctc ccagcaggag tgggagagtc tggactcttc ccagaggggc
      121 ttgtacagag atgtgatgtt ggagaactac aggaacttgg tgtcaatggc aggacattcc
      181 cgttctaaac cacatgtgat cgccttattg gaacaatgga aagagcctga agtgacagtg
      241 aggaaagatg gaagaagatg gtgcacagat ttgcagttgg aagatgatac aatcggctgt
      301 aaagaaatgc ccacctctga aaactgtcca tcttttgctc tacatcagaa aataagtaga
      361 cagaaaccac gtgaatgtca ggaatatgga aagacccttt gtcaagactc aaagcctgtt
      421 caacatgaaa gaatacatag tagtgaaaaa cccaacagat gtaaagaatg tgggaagaac
      481 tttagtaatg gacatcaact caccatacat cagagattgc atgttggtga gaaaccctat
      541 aaatatgaaa aatgtgggaa ggcctttatc agtggctcag cctttgttaa gcatgggaga
      601 attcacactg gtgagaagcc actcaaatgt aagcaatgtg gaaagactat tagtggtagc
      661 tatcaactta cagtacataa gagtattcat actgggaaga aaccatatga gtgcggggaa
      721 tgtgggaaag cttttctagt atatggaaag cttacccggc atcagagtac tcacactggt
      781 gaaaaaccct ttgggtgtga ggagtgtggg aaggccttca gtaccttttc atacctggtt
      841 caacatcagc gaattcatac cagtgaaaaa ccttacgaat gcaaagaatg tgggaaggcc
      901 tttagcacta gctcacccct tgctaagcat cagagaattc atactggcga gaaaccctat
      961 gaatgtaagg agtgtgggaa gtccttcact gtgtatggac agcttactcg acatcagagt
     1021 attcatactg gtgagaaacc ttttgaatgt aaggaatgtg gaaaggcctt tagacttagt
     1081 tccttccttc atgcacatca gcgaattcat gcagagataa agccctacgg atgcaaggaa
     1141 tgcgggagaa ccttcagtcg tgcctcatat cttgttcaac atggaagact tcacactggc
     1201 gagaagccct atgaatgtaa ggagtgtggc aaggccttta gtactggctc ataccttgtt
     1261 cagcatcaga ggatccatac tggggagaaa ccctatgaat gtaaggaatg tggcaaagcc
     1321 tttattagtc gccatcagct taccgtacat caaagggttc atactggaga gaaaccctat
     1381 gagtgtaagg aatgtggcaa ggccttcaga gtgcacgtac atctcacaca gcatcggaaa
     1441 attcatactg atgtaaagcc ctatgaatgt aaggaatgtg gaaagacttt tagtcgagcc
     1501 tcgtaccttg tacaacatag cagaatccat actggtaaga agccctatga gtgtaaggag
     1561 tgtggcaagg ccttcagttc tggctcatac cttgttcagc atcaaagaat tcatactggg
     1621 gagaaaccct atgaatgtaa caaatgtggg aaagccttta ctgtttatgg acaacttatt
     1681 ggacatcaga gtgttcacac tggtgagaaa ccttttgaat gtaaggaatg cgggaaggcc
     1741 tttagactta attcattcct tactgaacat cagcgggtac acactggtga gaaacccttt
     1801 aaatgcaaaa aatgtgggaa gacctttaga tacagttcag cccttaaagt gcatctgaga
     1861 aaacatatga gtgttatacc ctagtaatga actagtccgt aacttgaaag tatttcgatt
     1921 tcttggcttt atatatcttg tggaaaggac gaaacctagc ttgctaccca gcgtcg
//
