LOCUS       sequence-281135-        4691 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2818
FEATURES             Location/Qualifiers
     source          1..4691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgttgccctg taagaagaga agaactacag tgacagagtc cctacagcat
       61 aaaggcaatc aagaggaaaa caacgtagac ctagaatcag ccgttaaacc agaatctgac
      121 caggttaagg acttgagttc ggtgtcacta tcctgggatc caagtcatgg cagagtagct
      181 ggcttcgaag tacagtcttt gcaggatgca ggaaatcagc ttggtatgga ggatacatct
      241 ctgagctctg gaatgctcac ccagaacaca aatgtaccaa ttctagaagg tgttgatgtg
      301 gccatctctc agggaatcac cctaccttcc ttggagtctt ttcaccccct taatatacac
      361 attggtaaag gaaaactcca cgctactggc tcaaagagag ggaaaaaaat gacactcagg
      421 cctgggccag ttacccaaga agacagatgt gatcatctta ccctaaagga gcctttttca
      481 ggagagccta gtgaagaagt caaggaagaa ggagggaaac ctcaaatgaa ttctgaaggg
      541 gagatacctt ccctgccatc aggcagccaa tctgcaaaac cagtaagcca gcccaggaaa
      601 tcaacccagc cagatgtttg tgcctctcct caagaaaagc cactcaggac tctgtttcac
      661 caacctgagg aagagataga agatggtgga ctcttcattc caatggaaga acaagacaat
      721 gaagaaagtg agaaaaggag aaaaaagaaa aagggtacca agaggaaacg agatggaagg
      781 ggtcaagaag ggaccttggc atatgacctg aaactggatg acatgcttga ccgtaccttg
      841 gaggatggtg ccaagcagca caatctaaca gcagtcaatg tccgaaacat ccttcatgaa
      901 gtaatcacaa atgaacacgt ggtagctatg atgaaagcag ccatcagtga gacggaagat
      961 atgccaatgt ttgagcctaa aatgacacgc tctaaactga aggaagtagt ggaaaaagga
     1021 gtggtaattc caacatggaa tatttcacca attaagaagg ccaatgaaat taagcctcct
     1081 cagtttgtgg atatccacct tgaagaagat gattcctcag atgaagaata ccagccggat
     1141 gatgaagaag aagatgaaac tgctgaagag agcttattgg aaagtgatgt tgaaagcact
     1201 gcttcatctc cacgtggggc aaagaaatcc agattgaggc agtcttctga gatgactgaa
     1261 acagatgagg agagtggcat attatcagag gctgagaaag tcaccacacc agccatcagg
     1321 cacatcagtg ctgaggtagt gcccatgggg cccccgcccc ctccaaagcc gaaacagacc
     1381 agagatagta ctttcatgga gaagttacat gcggtagatg aggagctggc ttccagtcca
     1441 gtctgcatgg attctttcca gcccatggat gacagtctca ttgcatttcg aacgcgttct
     1501 aagatgcccc tgaaagatgt tcccctgggc caattagagg cagagctcca agctccagac
     1561 atcactccag atatgtatga ccccaatacg gcagatgatg aggactggaa gatgtggctg
     1621 gggggactta tgaatgatga tgtggggaat gaagatgaag cagatgatga tgatgatcca
     1681 gaatataatt tcctggaaga cctcgatgaa ccagacacag aggatttccg gactgaccgg
     1741 gcagtgagaa tcaccaaaaa ggaagtaaat gagctgatgg aagagctgtt tgaaactttc
     1801 caagatgaga tgggattctc caacatggaa gatgatggcc cagaagagga ggagtgtgta
     1861 gctgagcctc gtcctaactt taacacccct caagctctac ggtttgagga accactggcc
     1921 aacctgttaa atgaacaaca tcggacagtg aaggagctat ttgaacagct gaagatgaag
     1981 aaatcttcag ccaaacagct gcaggaagta gagaaggtta aaccccagag tgagaaagtt
     2041 catcagactc tgattctgga cccagcacag aggaagagac tccagcagca gatgcagcag
     2101 cacgttcagc tcttgaccca aatccacctt cttgccacct gcaaccccaa cctcaatccg
     2161 gaggccacta ccaccaggat atttcttaaa gagctgggaa cctttgctca aagctccatc
     2221 gcccttcacc atcagtacaa ccccaagttt cagaccctgt tccaaccctg taacttgatg
     2281 ggagctatgc agctgattga agacttcagc acacatgtca gcattgactg cagccctcat
     2341 aaaactgtca agaagactgc gaatgaattt ccctgtttgc caaagcaagt ggcttggatt
     2401 ctggccacaa gcaaggtttt catgtatcca gagttacttc cagtgtgttc cctgaaggca
     2461 aagaatcccc aggataagat cgtcttcacc aaggctgagg acaatttgtt agctttagga
     2521 ctgaagcatt ttgaaggaac tgagtttcct aatcctctaa tcagcaagta ccttctaacc
     2581 tgcaaaactg cccaccaact gacagtgaga atcaagaacc tcaacatgaa cagagctcct
     2641 gacaacatca ttaaatttta taagaagacc aaacagctgc cagtcctagg aaaatgctgt
     2701 gaagagatcc agccacatca gtggaagcca cctatagaga gagaagaaca ccggctccca
     2761 ttctggttaa aggccagtct gccatccatc caggaagaac tgcggcacat ggctgatggt
     2821 gctagagagg taggaaatat gactggaacc actgagatca actcagatcg aagcctagaa
     2881 aaagacaatt tggagttggg gagtgaatct cggtacccac tgctattgcc taagggtgta
     2941 gtcctgaaac tgaagccagt tgccacccgt ttccccagga aggcttggag acagaagcgt
     3001 tcatcagtcc tgaagcccct ccttatccaa cccagcccct ctctccagcc cagcttcaac
     3061 cctgggaaaa caccagcccg atcaactcat tcagaagccc ctccgagcaa aatggtgctc
     3121 cggattcctc acccaataca gccagccact gttttacaga cagttccagg tgtccctcca
     3181 ctgggggtca gtggaggtga gagttttgag tctcctgcag cactgcctgc tgtgccccct
     3241 gaggccagga caagcttccc tctgtctgag tcccagactt tgctctcttc tgcccctgtg
     3301 cccaaggtaa tgctgccctc ccttgcccct tctaagtttc gaaagccata tgtgagacgg
     3361 agaccctcaa agagaagagg agtcaaggcc tctccctgta tgaaacctgc ccctgttatc
     3421 caccaccctg catctgttat cttcactgtt cctgctacca ctgtgaagat tgtgagcctt
     3481 ggcggtggct gtaacatgat ccagcctgtc aatgcggctg tggcccagag tccccagact
     3541 attcccatca ctaccctctt ggttaaccct acttccttcc cctgtccatt gaaccagtcc
     3601 cttgtggcct cctctgtctc acccttaatt gtttctggca attctgtgaa tcttcctata
     3661 ccatccaccc ctgaagataa ggcccacgtg aatgtggaca ttgcttgtgc tgtggctgat
     3721 ggggaaaatg cctttcaggg cctagaaccc aaattagagc cccaggaact atctcctctc
     3781 tctgctactg ttttcccgaa agtggaacat agcccagggc ctccactagc agatgcagag
     3841 tgccaagaag gattgtcaga gaatagtgcc tgtcgctgga ccgttgtgaa aacagaggag
     3901 gggaggcaag ctctggagcc gctccctcag ggcatccagg agtctctaaa caaccctacc
     3961 cctggggatt tagaggaaat tgtcaagatg gaacctgaag aagctagaga ggaaatcagt
     4021 ggatcccctg agcgtgatat ttgtgatgac atcaaagtgg aacatgctgt ggaattggac
     4081 actggtgccc caagcgagga gttgagcagt gctggagaag taacgaaaca gacagtctta
     4141 cagaaggaag aggagaggag tcagccaact aaaacccctt catcttctca agagccccct
     4201 gatgaaggaa cctcagggac agatgtgaac aaaggatcat caaagaatgc tttgtcctca
     4261 atggatcctg aagtgaggct tagtagcccc ccagggaagc cagaagattc atccagtgtt
     4321 gatggtcagt cagtggggac tccagttggg ccagaaactg gaggagagaa gaatgggcca
     4381 gaagaagagg aagaagagga ctttgatgac ctcacccaag atgaggaaga tgaaatgtca
     4441 tcagcttctg aggaatctgt gctttctgtc ccagaactcc aggtgagagc tggagaatat
     4501 tctcaagtat ttcgtggact cagtaatatg tatcacttat tgatatgcca cctgcttgct
     4561 tgctgcacta tggatagtcc taaaatcatt tgtatttagt aatgaactag tccgtaactt
     4621 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacct tgcaacctag
     4681 ggaataacaa a
//
