LOCUS       sequence-281186-        1676 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0223
FEATURES             Location/Qualifiers
     source          1..1676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctaaaag accaggaccc cctggaagcc gagaaatggg actgttgaca
       61 ttcagagaca tagctataga attctctctg gaggaatggc aatgcctgga ttgtgctcag
      121 cggaatttat atagagatgt gatgttagag aactacagaa acctggtctc cctgggtatt
      181 gctgtctcta agccagactt gatcacctgt ctggagcaaa ataaagagtc ccagaatata
      241 aagagaaatg agatggtagc caaacaccca gttacgcgtt cccatttcac ccaagacctt
      301 cagccagagc agggcatcaa agattcactc caaaaagtaa taccaagaac atatggaaaa
      361 tgtggacatg agaaattaca atttaaaaaa tgctgtaaaa gtgtgggtga atatgaggtg
      421 cacaagggag gttatagtga agttaaccaa tgtttgtcaa ctacccaaaa caaaatattt
      481 cagactcata aatatgtcaa agtctttggt aaattttcaa attccaatag agataaaaca
      541 agatatactg gaaataaaca tttcaaatgt aacaaatatg gcaaatcatt ttgcatgctt
      601 tcacacctaa atcaacatca ggtaattcat actagggaga agtcctacaa atgcaaagaa
      661 tgtggcaaat cctttaactg ctcctcaaac catactacac ataaaataat tcatactgga
      721 gagaaaccat atagatgtga ggaatgtggc aaagccttta gctggtctgc aaaccttact
      781 agacataaga gaactcatac tggagagaaa ccctacacgt gtgaagaatg tggccaagcc
      841 tttaggcgct cctcagcact tactaaccac aagagaattc atactggaga gagaccctac
      901 aaatgtgaag aatgtggcaa agcctttagc gtatcctcaa ccctcactga ccacaagaga
      961 attcatactg gagagaaacc ctgcaggtgt gaggaatgtg gcaaagcctt tagctggtcc
     1021 tcaaacctta ctagacataa gagaattcat actagagaga aaccctatgc ctgtgaagaa
     1081 tgtggccaag cctttagctt atcctcgaac cttatgagac ataggagaat tcatactgga
     1141 gagaaaccct acacatgtga agaatgtggc caagacttta ggcgctcctc agcacttact
     1201 atccacaaga gaattcatac tggagagaga ccctacaaat gtgaagagtg tggcaaagtc
     1261 tttagcttat cctcaaccct cactgaccac aagagaattc atactggaga gagaccctac
     1321 aaatgtgaag aatgtggcaa agcctttagc ttatcctcaa ccctcactga ccacaagaga
     1381 attcatactg gagagagacc ctacacatgt gaagaatgtg gcaaagcctt taattgctcc
     1441 tcaaccctta tgcaacataa gagaattcat actggagaga aaccctacaa atgtgaagaa
     1501 tgtgagcaag cttttaagtg gcattcaagt cttgctaaac ataagataat tcacactgga
     1561 gagaaaccct acaaatgtga atagtaatga actagtccgt aacttgaaag tatttcgatt
     1621 tcttggcttt atatatcttg tggaaaggac gatactcacg ccaggtcagg gagctc
//
