LOCUS       sequence-281195-        3656 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0345
FEATURES             Location/Qualifiers
     source          1..3656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtgga caccgaggag aagcggcatc gcacgcggtc caaaggggtt
       61 cgagttcccg tggaaccagc catacaagag ctgttcagct gtcccacccc tggctgtgac
      121 ggcagtggtc atgtcagtgg caaatatgca agacacagaa gtgtatatgg ttgtcccttg
      181 gcgaaaaaaa gaaaaacaca agataaacag ccccaggaac ctgctcctaa acgaaagcca
      241 tttgccgtga aagcagacag ctcctcagtg gatgagtgtg acgacagtga tgggactgag
      301 gacatggatg agaaggagga ggatgagggg gaggagtact ccgaggacaa tgatgagcca
      361 ggggatgagg acgaggagga cgaggagggg gaccgggagg aggaggagga gatcgaggag
      421 gaggatgagg acgatgacga ggatggagaa gatgtggagg atgaagaaga ggaagaggag
      481 gaggaggagg aggaggaaga ggaagaagaa aacgaagacc atcaaatgaa ttgtcacaat
      541 actcgaataa tgcaagacac agaaaaggat gataacaata atgacgaata tgacaattac
      601 gatgaactgg tggccaagtc attgttaaac ctcggcaaaa tcgctgagga tgcagcctac
      661 cgggccagga ctgagtcaga aatgaacagc aatacctcca atagtctgga agacgatagt
      721 gacaaaaacg aaaacctggg tcggaaaagt gagttgagtt tagacttaga cagtgatgtt
      781 gttagagaaa cagtggactc ccttaaacta ttagcccaag gacacggtgt tgtgctctca
      841 gaaaacatga atgacagaaa ttatgcagac agcatgtcgc agcaagacag tagaaatatg
      901 aattacgtca tgttggggaa gcccatgaac aacggactca tggaaaagat ggtggaggag
      961 agcgatgagg aggtgtgtct gagcagtctg gagtgtttga ggaatcagtg cttcgacctg
     1021 gccaggaagc tcagtgagac caacccgcag gagaggaatc cgcagcagaa catgaacatc
     1081 cgtcagcatg tccggccaga agaggacttc cccggaagga cgccggacag aaactactcg
     1141 gacatgctga acctcatgcg gctggaggag cagttgagcc cccggtcgag agtgtttgcc
     1201 agctgtgcga aggaggatgg gtgtcatgag cgggacgacg ataccacctc tgtgaactcg
     1261 gacaggtctg aagaggtgtt cgacatgacc aaggggaacc tgaccctgct ggagaaagcc
     1321 atcgctttgg aaacggaaag agcaaaggcc atgagggaga agatggccat ggaagctggg
     1381 aggagggaca atatgaggtc atatgaggac cagtctccga gacaacttcc cggggaggac
     1441 agaaagccta aatccagtga cagccatgtc aaaaagccat actatgatcc ctcaagaaca
     1501 gaaaagaaag agagcaagtg tccaaccccc gggtgtgatg gaaccggcca cgtaactggg
     1561 ctgtacccac atcaccgcag cctgtccgga tgcccgcaca aagatagggt ccctccagaa
     1621 atccttgcca tgcatgaaag tgtcctcaag tgccccactc cgggctgcac ggggcgcggg
     1681 catgtcaaca gcaacaggaa ctcccaccga agcctctccg gatgcccgat cgctgcagca
     1741 gagaaactgg ccaaggcaca ggaaaagcac cagagctgcg acgtgtccaa gtccagccag
     1801 gcctcggacc gcgtgctcag gccaatgtgc tttgtgaagc agctggagat tcctcagtat
     1861 ggctacagaa acaatgtccc cacaactacg ccgcgttcca acctggccaa ggagctcgag
     1921 aaatattcca agacctcgtt tgaatacaac agttacgaca accatactta tggcaagcga
     1981 gccatagctc ccaaggtgca aaccagggat atatccccca aaggatatga tgatgcgaag
     2041 cggtactgca aggaccccag ccccagcagc agcagcacca gcagctacgc gcccagcagc
     2101 agcagcaacc tgagctgcgg cgggggcagc agcgccagca gcacgtgcag caagagcagc
     2161 ttcgactaca cgcacgacat ggaggcggcc cacatggcgg ccaccgccat cctcaacctg
     2221 tccacgcgct gccgcgagat gccgcagaac ctgagcacca agccgcagga cctgtgcgcc
     2281 acgcggaacc ctgacatgga ggtggatgag aacgggaccc tggacctcag catgaacaag
     2341 cagaggccgc gggacagctg ctgccccatc ctgacccctc tggagcccat gtccccccag
     2401 cagcaggcag tgatgaacaa ccggtgtttc cagctgggcg agggcgactg ctgggacttg
     2461 cccgtagact acaccaaaat gaaaccccgg aggatagacg aggacgagtc caaagacatt
     2521 actccagaag acttggaccc attccaggag gctctagaag aaagacggta tcccggggag
     2581 gtgaccatcc caagtcccaa acccaagtac cctcagtgca aggagagcaa aaaggactta
     2641 ataactctgt ctggctgccc cctggcggac aaaagcattc gaagtatgct ggccaccagc
     2701 tcccaagaac tcaagtgccc cacgcctggc tgtgatggtt ctggacatat caccggcaat
     2761 tatgcttctc atcggagcct ttcaggttgc ccaagagcaa agaaaagtgg tatcaggata
     2821 gcacagagca aagaagataa agaagatcaa gaacccatca ggtgtccggt ccccgggtgc
     2881 gacggccagg gccacatcac tgggaagtac gcgtcccatc gcagcgcctc cgggtgcccc
     2941 ttggcggcca agaggcagaa agacgggtac ctgaatggct cccagttctc ctggaagtcg
     3001 gtcaagacgg aaggcatgtc ctgccccacg ccaggatgcg acggctcagg ccacgtcagc
     3061 ggcagcttcc tcacacaccg cagcttgtca ggatgcccga gagccacgtc agcgatgaag
     3121 aaggcaaagc tttctggaga gcagatgctg accatcaaac agcgggccag caacggtata
     3181 gaaaatgatg aagaaatcaa acagttagat gaagaaatca aggagctaaa tgaatccaat
     3241 tcccagatgg aagccgatat gattaaactc agaactcaga ttaccacgat ggagagcaac
     3301 ctgaagacca tcgaagagga gaacaaagtg attgagcagc agaacgagtc tctcctccac
     3361 gagctggcga acctgagcca gtctctgatc cacagcctgg ctaacatcca gctgccgcac
     3421 atggatccaa tcaatgaaca aaattttgat gcttacgtga ctactttgac ggaaatgtat
     3481 acaaatcaag atcgttatca gagtccagaa aataaagccc tactggaaaa tataaagcag
     3541 gctgtgagag gaattcaggt ctagtaatga actagtccgt aacttgaaag tatttcgatt
     3601 tcttggcttt atatatcttg tggaaaggac gatgctctgc ttgtacctag tgcgtt
//
