LOCUS       sequence-281291-        1454 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0592
FEATURES             Location/Qualifiers
     source          1..1454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatccggg gaatgagaat tcagccacag aggctgccgc gatcatagac
       61 ctagatcccg acttcgaacc ccagagccgt ccccgctcct gcacctggcc ccttccccga
      121 ccagagatcg ctaaccagcc gtccgagccg cccgaggtgg agccagatct gggggaaaag
      181 gccattgaaa gcgccccgga gaagcgactg acacttgccc agatctacga gtggatggtc
      241 cgtactgtac cctacttcaa ggacaagggt gacagcaaca gctcagcagg atggaagaac
      301 tcgatccgcc acaacctgtc cctgcacagc aagttcatca aggttcacaa cgaggccacc
      361 ggcaaaagct cttggtggat gctgaaccct gagggaggca agagcggcaa agccccccgc
      421 cgccgggccg cctccatgga tagcagcagc aagctgctcc ggggccgcag taaagccccc
      481 aagaagaaac catctgtgct gccagctcca cccgaaggtg ccactccaac gagccctgtc
      541 ggccactttg ccaagtggtc aggcagccct tgctctcgaa accgtgaaga agccgatatg
      601 tggaccacct tccgtccacg aagcagttca aatgccagca gtgtcagcac ccggctgtcc
      661 cccttgaggc cagagtctga ggtgctggcg gaggaaatac cagcttcagt cagcagttat
      721 gcagggggtg tccctcccac cctcaatgaa ggtctagagc tgttagatgg gctcaatctc
      781 acctcttccc attccctgct atctcggagt ggtctctctg gcttctcttt gcagcatcct
      841 ggggttaccg gccccttaca cacctacagc agctcccttt tcagcccagc agaggggccc
      901 ctgtcagcag gagaagggtg cttctccagc tcccaggctc tggaggccct gctcacctct
      961 gatacgccac caccccctgc tgacgtcctc atgacccagg tagatcccat tctgtcccag
     1021 gctccgactc ttctgttgct gggggggctt ccttcctcca gtaagctggc cacgggcgtc
     1081 ggcctgtgtc ccaagcccct agaggctcca ggccccagca gtctggttcc caccctttct
     1141 atgatagcac cacctccagt catggcaagt gcccccatcc ccaaggctct ggggactcct
     1201 gtgctcacac cccctactga agctgcaagc caagacagaa tgcctcagga tctagatctt
     1261 gatatgtata tggagaacct ggagtgtgac atggataaca tcatcagtga cctcatggat
     1321 gagggcgagg gactggactt caactttgag ccagatccct agtaatgaac tagtccgtaa
     1381 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ggtgtatcac
     1441 gtaactctgt ctaa
//
