LOCUS       sequence-281459-        1046 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1403
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaaaact cacaagacgt gattttcagt gctccagttt cagagatgat
       61 tgggaatgta atcggcagtt taagaaagaa ctcggctctc aggggggaca tttcaatcaa
      121 ttggtattca ctcatgaaga tctgcccact ttgagtcacc atccatcctt cacattacag
      181 caaatcatta acagtaaaaa gaaattctgt gcatctaaag aatataggaa aacctttaga
      241 catggctcac agtttgctac acatgagata attcatacca ttgagaagcc ttatgaatgt
      301 aaggaatgtg gaaagtcctt tagacatccc tcaagactca ctcatcatca gaaaattcat
      361 actggcaaga aaccctttga atgtaaggaa tgtggaaaaa cctttatttg tggctcagac
      421 cttactcgac atcacagaat tcacactggt gagaaaccct atgaatgtaa ggaatgtggg
      481 aaagccttta gtagtggttc aaacttcact cgacatcaga gaattcacac aggtgagaag
      541 ccttatgaat gcaaagaatg cgggaaggcc tttagtagtg gctcaaactt tactcaacat
      601 cagagaattc atactgggga aaaaccctat gaatgtaagg aatgtggcaa tgcctttagt
      661 cagagctcac aacttattaa acatcaaaga atccatacag gtgagaaacc ttacgaatgt
      721 aaggaatgtg aaaaggcttt tcgttctggc tcagacctta ctagacatca gagaattcat
      781 actggggaga aaccctatga atgtaagata tgtgggaagg cttattctca gagttcacag
      841 cttattagtc atcatagaat tcatactagt gagaaaccct atgaatatag ggaatgtgga
      901 aagaacttta attatgaccc acaacttatt cagcatcaaa atttgtactg gtagtaatga
      961 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1021 gacttaaaca ccgggcctat ccattc
//
