LOCUS       sequence-281615-        2465 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1826
FEATURES             Location/Qualifiers
     source          1..2465
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgac tactgccaac cccgaaatga catcagatgt accatcactg
       61 ggtccagcca ttgcctctgg aaactctgga cctggaattc aaggtggagg agccattgtc
      121 cagagggcta ttaagcggcg accagggctg gattttgatg atgatggaga agggaacagt
      181 aaatttttga ggtgtgatga tgatcagatg tctaacgata aggagcggtt tgccaggtcg
      241 gatgatgagc agagctctgc ggataaagag agacttgcca gggaaaatca cagtgaaatt
      301 gaacggcggc gacggaacaa gatgacagcc tacatcacag aactgtcaga tatggtaccc
      361 acctgtagtg ccctggctcg aaaaccagac aagctaacca tcttacgcat ggcagtttct
      421 cacatgaagt ccttgcgggg aactggcaac acatccactg atggctccta taagccgtct
      481 ttcctcactg atcaggaact gaaacatttg atcttggagg cagcagatgg ctttctgttt
      541 attgtctcat gtgagacagg cagggtggtg tatgtgtctg actccgtgac tcctgttttg
      601 aaccagccac agtctgaatg gtttggcagc acactctatg atcaggtgca cccagatgat
      661 gtggataaac ttcgtgagca gctttccact tcagaaaatg ccctgacagg gcgtatcctg
      721 gatctaaaga ctggaacagt gaaaaaggaa ggtcagcagt cttccatgag aatgtgtatg
      781 ggctcaagga gatcgtttat ttgccgaatg aggtgtggca gtagctctgt ggacccagtt
      841 tctgtgaata ggctgagctt tgtgaggaac agatgcagga atggacttgg ctctgtaaag
      901 gatggggaac ctcacttcgt ggtggtccac tgcacaggct acatcaaggc ctggccccca
      961 gcaggtgttt ccctcccaga tgatgaccca gaggctggcc agggaagcaa gttttgccta
     1021 gtggccattg gcagattgca ggtaactagt tctcccaact gtacagacat gagtaatgtt
     1081 tgtcaaccaa cagagttcat ctcccgacac aacattgagg gtatcttcac ttttgtggat
     1141 caccgctgtg tggctactgt tggctaccag ccacaggaac tcttaggaaa gaatattgta
     1201 gaattctgtc atcctgaaga ccagcagctt ctaagagaca gcttccaaca ggtagtgaaa
     1261 ttaaaaggcc aagtgctgtc tgtcatgttc cggttccggt ctaagaacca agaatggctc
     1321 tggatgagaa ccagctcctt tactttccag aacccttact cagatgaaat tgagtacatc
     1381 atctgtacca acaccaatgt gaagaactct agccaagaac cacggcctac actctccaac
     1441 acaatccaga ggccacaact aggtcccaca gctaatttac ccctggagat gggctcagga
     1501 cagctggcac ccaggcagca gcaacagcaa acagaattgg acatggtacc aggaagagat
     1561 ggactggcca gctacaatca ttcccaggtg gttcagcctg tgacaaccac aggaccagaa
     1621 cacagcaagc cccttgagaa gtcagatggt ttatttgccc aggatagaga tccaagattt
     1681 tcagaaatct atcacaacat caatgcggat cagagtaaag gcatctcctc cagcactgtc
     1741 cctgccaccc aacagctatt ctcccagggc aacacattcc ctcctacccc ccggccggca
     1801 gagaatttca gtggcctagc ccctcctgta accattgtcc agccatcagc ttctgcagga
     1861 cagatgttgg cccagatttc ccgccactcc aaccccaccc aaggagcaac cccaacttgg
     1921 acccctacta cccgctcagg cttttctgcc cagcaggtgg ctacccaggc tactgctaag
     1981 actcgtactt cccagtttgg tgtgggcagc tttcagactc catcctcctt cagctccatg
     2041 tccctccctg gtgccccaac tgcatcgcct ggtgctgctg cctaccctag tctcaccaat
     2101 cgtggatcta actttgctcc tgagactgga cagactgcag gacaattcca gacacggaca
     2161 gcagagggtg tgggtgtctg gccacagtgg cagggccagc agcctcatca tcgttcaagt
     2221 tctagtgagc aacatgttca acaaccgcca gcacagcaac ctggccagcc tgaggtcttc
     2281 caggagatgc tgtccatgct gggagatcag agcaacagct acaacaatga agaattccct
     2341 gatctaacta tgtttccccc cttttcagaa tagtaatgaa ctagtccgta acttgaaagt
     2401 atttcgattt cttggcttta tatatcttgt ggaaaggacg aagcgtggcg aggcgatccg
     2461 cgagg
//
