LOCUS       sequence-281951-        2270 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1431
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggctc acgtccccgc gccccgagcg gccacttagc gccgtcgccg
       61 ccggctttcg acggcgagct ggatctgcag cgatactcca acgggccagc cgtgagcgca
      121 gggtcgctcg ggatgggagc ggtgagctgg tctgagagtc gtgcaggcga acggcgcttc
      181 ccctgccctg tatgcgggaa gcgcttccgc ttcaactcta tccttgcttt gcacctgcgg
      241 gcgcacccag gagcccaggc cttccagtgc cctcactgcg gccaccgcgc ggcgcagcgg
      301 gctctgctgc gctcgcacct gcgcacacac cagcccgagc gcccacgtag tcctgctgca
      361 cgcctgttgc tggagttgga agagcgcgcg ctactacgcg aggcccgact ggggagagcc
      421 cgaagctcag ggggcatgca ggccacccct gccactgagg gtctggcgcg gccccaggct
      481 ccttcatcgt ccgccttccg ttgcccctac tgcaaaggca agtttcgcac ctcggcggag
      541 cgcgaacgcc acctgcacat cctgcatagg ccctggaagt gcggcctgtg cagtttcggc
      601 tccagccagg aggaggagct gctgcaccac agcctgacgg cccacggggc tcccgagcgt
      661 cccctggcgg ccacctccgc tgcgcctccg cctcagcctc agcctcagcc tccaccccag
      721 cccgaaccca gatcagtccc ccagccggag ccggagccgg agcccgaacg tgaggcaacc
      781 ccgaccccag ctcctgccgc tcccgaggag cccccagcgc ctccggagtt ccgctgccaa
      841 gtgtgcggcc agagctttac acagtcttgg tttctcaagg gccacatgcg taagcacaag
      901 gcctccttcg atcatgcgtg tccggtgtgc ggccgctgct tcaaggagcc ctggttcctt
      961 aagaaccaca tgaaggtgca cgccagcaag ctgggcccac tgcgtgcccc ggggcctgcc
     1021 tccgggcctg cccgcgcccc ccagcctcct gacctcggcc tgctggccta tgagccgttg
     1081 ggcccagcgc tcctcttggc cccggctccc accccggccg agcgccgtga gcccccgagc
     1141 ctcttgggct acctgagcct gcgagctggc gagggccggc ccaacggcga gggtgctgag
     1201 cccggtcccg gccgcagctt cggaggcttc cgcccgctgt cctctgctct cccggcccgg
     1261 gctcgccggc accgtgcgga ggagcctgag gaagaagagg aggtggtgga ggccgaggag
     1321 gaaacctggg cccggggcag gtcgctgggc tctctggctt ccctgcaccc gcgcccgggt
     1381 gagggaccgg ggcactctgc ctctgctgct ggggcccagg caagatcgac cgccacgcag
     1441 gaagagaatg ggctgttggt tggagggacc cggcctgaag ggggccgggg cgccaccggc
     1501 aaggattgtc ctttctgcgg aaaatctttc cgctcagcac atcacctcaa agtgcatctg
     1561 cgagtgcaca caggcgagcg gccctacaag tgtccgcact gcgactacgc gggcacccag
     1621 tccggctcgc tcaagtatca cctacagcgc caccaccggg agcagaggag cggggccggc
     1681 cccgggccac ccccggagcc accgcctcct tcccagcggg gttcggcccc gcaatctgga
     1741 gccaagccgt ctccgcagcc tgcgacctgg gtggagggcg cctcaagtcc ccggcctcct
     1801 tctagcggtg ctgggccggg gtcccgtcgg aagcccgcca gccctgggag gaccctgcgc
     1861 aacgggcgag gcggtgaggc cgaacccctg gacctgtcct tgcgggcagg gccgggaggc
     1921 gaggccgggc ctgggggtgc cctccaccgc tgcctcttct gcccgttcgc cactggagcc
     1981 ccagagctca tggccttgca ccttcaagtg caccacagcc gccgggctag gggccgccgg
     2041 ccaccccagg ctgacgcgtc cccgccctat gcccgagtac catcaggaga gacccctccc
     2101 agtccttcgc aggaagggga ggagggctcc gggctgtcca gacccggaga ggcagggctg
     2161 ggggggcaag aacggtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2221 ctttatatat cttgtggaaa ggacgagcca tggagcgtac cggaggttcc
//
