LOCUS       sequence-282035-        1430 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1588
FEATURES             Location/Qualifiers
     source          1..1430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cacgctgagg gaccccgctc agcagggcta tgtgaccttt
       61 gaggacgtgg ctgtctactt ctcccaggag gaatggagat tgcttgatga cgctcagagg
      121 ctcctctacc gcaatgtgat gctggagaac tttacacttc tggcctctct gggacttgca
      181 tcttccaaga cccatgaaat aacccagctg gagtcatggg aggagccctt catgcctgct
      241 tgggaagttg tgacttcagc catactgaga ggtagttggc aaggagccaa ggctgaggca
      301 gctgctgagc agagtgcttc tgtagaagtg cccagttcaa acgttcagca acaccagaag
      361 cagcactgtg gagagaaacc cttaaaaaga caagagggca gggtcccagt tttgaggagt
      421 tgcagagtcc acctatcaga gaagtccttg caaagcaggg aagttgggaa ggatcttctg
      481 accagctcag gtgttctcaa gcaccaggtg actcacacgg gagagaagtc acataggagc
      541 tccaaaagta gggaggcctt tcatgctgga aaaaggcatt acaaatgcag tgaatgtggg
      601 aaagcctttg gtcagaaata tttacttgtt cagcaccaga gactgcacac tggggaaaag
      661 ccttatgaat gcagtgaatg tgggaagtta tttagccata agtccaacct ttttatacac
      721 caaatagttc acactggaga aaggccttat gggtgtagtg actgtggaaa atcctttagc
      781 cgtaatgctg acctcattca acaccagaga gttcacactg gagaaaagcc ttttacatgc
      841 agtgaatgtg gaaaagcttt caggcataat tccacacttg ttcagcatca cagaatccac
      901 actggagtaa ggccttatga gtgcagtgaa tgtggaaaat tgtttagttt caactccagc
      961 ctcatgaaac atcagagagt tcacactgga gaaagacctt ataagtgcag tgaatgtgga
     1021 aaattctata gccacaagtc cagccttatc aatcattggc gtgttcacac tggagaaagg
     1081 ccttatgagt gcagtgaatg tgggaaattt tttagccaaa gctcaagcct catgcaacat
     1141 cgaaaagttc acactggaga aaaacctttt aagtgcaatg aatgtgggag attctttagt
     1201 gagaattcca gccttgttaa acatcagagg gttcacactg gagcaaagcc ttatgagtgc
     1261 agggaatgtg ggaaattttt tcgccacagc tccagtcttg ttaaacatcg aaggattcac
     1321 actggagaaa tacaatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1381 ctttatatat cttgtggaaa ggacgactgc agtcattgtg accacatagg
//
