LOCUS       sequence-282059-        1142 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1700
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..188
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
ORIGIN
        1 accgccacca tggcggcggc agagcccgcg agctcgggcc agcaggcgcc ggcagggcag
       61 gggcagggcc agcggccgcc gccgcagcct ccgcaggcgc aagccccgca gccgcccccg
      121 ccgccgcagc tcgggggcgc cgggggcggc agcagcaggc acgagaagag cctggggctg
      181 ctcactacca agttcgtgtc gctgctgcag gaggccaagg acggcgttct ggatctcaaa
      241 gcggctgctg atactttggc tgtgaggcaa aaaaggagaa tttatgatat caccaatgtc
      301 ttagagggaa ttgacttgat tgaaaaaaag tcaaaaaaca gtatccagtg gaaaggtgta
      361 ggtgctggct gtaatactaa agaagtcata gatagattaa gatatcttaa agctgaaatt
      421 gaagatctag aactgaagga aagagaactt gatcagcaga agttgtggct acagcaaagc
      481 atcaaaaatg tgatggacga ttccattaat aatagatttt cctatgtaac tcatgaagac
      541 atctgtaatt gctttaatgg tgatacactt ttggccattc aggcaccttc tggtacacaa
      601 ctggaggtac ccattccaga aatgggtcag aatggacaaa agaaatacca gatcaatcta
      661 aagagtcatt caggacctat ccatgtgctg cttataaata aagagtcgag ttcatctaag
      721 cccgtggttt ttcctgttcc cccacctgat gacctcacac agccttcctc ccagtccttg
      781 actccagtga ctccacagaa atccagcatg gcaactcaaa atctgcctga gcaacatgtc
      841 tctgaaagaa gccaggctct gcagcagaca tcagctacag atatatcttc agcaggatct
      901 attagtggag atatcattga tgagttaatg tcttctgacg tgtttcctct cttaaggctt
      961 tctcctaccc cggcagatga ctacaacttt aatttagatg ataacgaagg agtttgtgat
     1021 ctgtttgatg tccagatact aaattattag taatgaacta gtccgtaact tgaaagtatt
     1081 tcgatttctt ggctttatat atcttgtgga aaggacgacg atgactcggt tcaccactgt
     1141 at
//
