LOCUS       sequence-282200-        1664 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2207
FEATURES             Location/Qualifiers
     source          1..1664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtgct ggagagcggg gagcagggcg tgctgcagtg ggaccgcaag
       61 ctgagcgagc tgtcagagcc cggggacggc gaggccctca tgtaccacac gcacttctca
      121 gaacttctgg atgagttttc ccagaacgtc ttgggtcagc tcctgaatga tcctttcctc
      181 tcagagaaga gtgtgtcaat ggaggtggaa ccttccccga cgtccccggc gcctctcatc
      241 caggctgagc acagctactc cctgtgcgag gagcctcggg cccagtcgcc cttcacccac
      301 attaccacca gtgacagctt caatgacgat gaggtggaaa gtgagaaatg gtacctgtct
      361 acagacttcc cttcaacatc catcaagaca gagccagtta cagacgaacc acccccagga
      421 ctcgttccgt ctgtcactct gaccatcaca gccatctcca ccccgttgga aaaggaggaa
      481 cctcctctgg aaatgaacac tggggttgat tcctcgtgcc agaccattat tcctaaaatt
      541 aagctggagc ctcatgaagt ggatcagttt ctaaacttct ctcctaaaga agccccagtg
      601 gaccacctgc atttgccgcc cacccctccg agcagtcacg gcagtgactc agagggcagc
      661 ctgagtccca acccacgcct gcaccccttc agcctgcctc agacccacag cccctccaga
      721 gctgcacccc gggccccctc cgccctctcc agctcccctc tcctcacggc tcctcataaa
      781 ctgcagggat caggccctct ggtcctgaca gaggaggaga agaggaccct gatcgctgag
      841 ggctatccca tccccaccaa attgcccctg tcaaaatcag aggagaaggc cctgaagaaa
      901 attcggagga agatcaagaa taagatttct gctcaggaaa gtaggagaaa gaagaaagaa
      961 tacatggaca gcctggagaa aaaagtggag tcttgttcaa ctgagaactt ggagcttcgg
     1021 aagaaggtag aggttctaga gaacactaat aggactctcc ttcagcaact ccagaagctt
     1081 cagactttgg tgatgggcaa ggtttctcga acctgcaagt tagctggcac gcagactggc
     1141 acctgcctca tggttgtggt gctgtgcttt gccgttgcat tcggcagctt ctttcaaggc
     1201 tacgggccct atccttctgc caccaagatg gctctgccca gccagcattc cctgcaggag
     1261 ccctacacag cctccgtggt gagatccaga aacctgctga tctacgagga acattctccc
     1321 ccagaggagt catccagccc gggctcggct ggggagctgg ggggctggga tagaggttcc
     1381 tccctgctca gggtgtcagg gctggagtcc aggccggatg tggatcttcc ccatttcatt
     1441 atctcgaatg agaccagcct ggagaagtca gtgcttttgg agctgcagca gcacctggtc
     1501 agcgccaaac tggaggggaa tgaaacacta aaagttgtag aactcgacag aagagtgaac
     1561 accactttct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1621 atatcttgtg gaaaggacga cagctcgtgg gttcttacgt ggaa
//
