LOCUS       sequence-282422-        3584 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0685
FEATURES             Location/Qualifiers
     source          1..3584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3432..3449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggattccaa agatgaaagc tcgcatgtgt ggccgacatc tgcagagcat
       61 gaacagaatg ccgcacaggt gcactttgtt ccggacacag gaacagtggc tcagattgtc
      121 tataccgatg accaggttcg ccccccacag caggtggtgt acacggcaga tggtgcctcc
      181 tacacatcag tggatggtcc agagcacacg ctggtgtaca tccacccggt ggaagctgca
      241 cagactctgt ttacagaccc cggccaggta gcttatgtcc aacaggatgc tacagctcag
      301 caggcctcat tgccagttca taaccaggtg ctgccttcca tcgagagtgt agatgggtcc
      361 gaccctttgg caactctgca gacccctcta ggcagactgg aggccaaaga ggaagaggat
      421 gaggatgagg acgaggacac tgaggaagat gaggaagaag acggtgagga cacggatctg
      481 gatgactggg agccagaccc gccccggccc ttcgacccac acgacttgtg gtgtgaggag
      541 tgcaataacg cgcatgcttc agtgtgtccg aagcacggcc ccttgcaccc gatccccaac
      601 cggccggtgc tcacccgggc cagggcgagc ctccccctgg tgctctacat agacaggttt
      661 ctgggcgggg tgttctccaa gcggcgcatc cccaagcgca cccagtttgg ccccgtggag
      721 gggcctctcg tcaggggctc ggagctgaaa gactgttaca ttcacctcaa ggtttctctt
      781 gataaagggg acaggaaaga aagggattta catgaagacc tatggtttga gttgtctgat
      841 gagacgcttt gtaactggat gatgtttgta cggccagccc agaatcacct ggagcagaac
      901 ctggtggctt accagtatgg ccaccatgtg tattatacaa ccataaaaaa tgtggagccc
      961 aagcaggaac tgaaggtgtg gtatgccgca tcctatgctg agttcgtgaa ccagaaaatt
     1021 catgacattt ctgaggaaga aaggaaagtt cttcgagagc aagagaagaa ttggccctgc
     1081 tatgaatgta accgccgatt tataagctcg gagcagttgc aacagcatct caattctcat
     1141 gatgagaaac tagatgtgtt tagcagaaca agaggcagag gaaggggacg aggcaagagg
     1201 cgattcggtc caggtcgacg gccggggcgt cctccaaaat ttatccgcct ggaaatcacc
     1261 agcgaaaatg gggaaaagag tgacgatggg acacaggact tgctacattt tcccacaaag
     1321 gagcaatttg atgaggctga accagccact ctgaatgggc tggatcaacc agaacagacc
     1381 actatcccaa tccctcagct gccacaggaa acccagtctt ccctggaaca tgaaccagaa
     1441 actcacaccc tgcacctgca gccgcagcat gaagagagcg tggtgcccac ccagagcacg
     1501 ctgacagccg acgacatgcg cagagccaag cgcatccgat tggagctgca gaatgcagct
     1561 cttcagcatc tgtttattcg gaagtccttc cggcctttta aatgcttgca gtgtgggaag
     1621 gccttccggg aaaaggacaa actggaccag cacttacgct tccatgggcg ggaggggaac
     1681 tgcccactga cctgtgatct ctgtaacaag ggcttcatca gcagcacatc cttggagagc
     1741 cacatgaagc tccactcaga ccagaagact tactcttgca ttttttgccc agaatccttt
     1801 gaccgccttg atttgttgaa agatcatgtg gccattcata tcaatgatgg ctacttcacc
     1861 tgcccaactt gtaagaaacg gttcccagat tttatccagg tgaaaaaaca cgtgcgcagc
     1921 ttccactcag aaaagatcta ccagtgcaca gagtgtgaca aggccttctg tcgccccgat
     1981 aaactgcgac tccacatgct ccggcattcg gaccgcaaag acttcctgtg ttccacctgt
     2041 gggaagcaat ttaagcgaaa agacaaacta cgggaacaca tgcagaggat gcataatcct
     2101 gagagggagg ccaagaaagc cgaccgcatc agccgctcca agacgttcaa gccccgcatc
     2161 acgtccacag actacgacag cttcacgttc aagtgccgcc tgtgcatgat gggcttccgg
     2221 cggcgcggca tgctggtaaa tcacttatcg aagagacacc cagacatgaa gatagaagag
     2281 gtgccagagt taactctacc catcataaaa cccaatcgtg attacttttg tcagtattgc
     2341 gataaggttt ataaaagtgc cagcaagcgc aaagcccaca ttctgaagaa ccacccagga
     2401 gcagagctcc caccgagcat tcggaagctc cgacccgctg gtcctggaga gccagacccc
     2461 atgctgagca cacacaccca gctgacgggc accatcgcca cccctcccgt ctgctgtccc
     2521 cactgctcca agcagtacag cagcaagacc aagatggtcc agcacattcg aaagaagcat
     2581 ccagagttcg cccagctctc caacaccata cacacaccac tgacgacagc tgtgatcagt
     2641 gccaccccag cggttttgac tacagacagc gccactggag agactgtggt gacgacggac
     2701 ctgctcaccc aagcaatgac agaactgtcc cagaccttaa cgacagacta ccgaacgcca
     2761 caaggggatt accagagaat tcagtacatc cctgtgtcgc agtcggcgtc tggcctccag
     2821 cagcctcagc acatacagct gcaagtggtt caagtggcct cggccacttc ccctcaccag
     2881 tcacagcagt ccactgtgga tgttggccag ctccatgatc ctcagcccta cccccagcac
     2941 gccatccagg tgcagcacat ccaggtcagc gagcctaccg cctcggcccc gtcctccgcc
     3001 caggtatctg ggcagccgtt gagtccctca gcccagcagg ctcagcaggg gctcagcccc
     3061 tcccacatcc agggcagttc ttccacacag gggcaggctc tgcagcagca gcagcagcag
     3121 cagcagaatt cctctgtgca gcacacgtac ctgcccagtg cttggaattc cttccgtggc
     3181 tattcatctg agattcaaat gatgacgctt cctccgggtc agtttgtgat tacagacagt
     3241 ggtgtggcaa ctccagttac tactggccag gtgaaggcgg ttacttcggg tcattatgtg
     3301 ttatcagaaa gtcaatcaga attggaagaa aagcaaactt ctgccctctc tggtggagtc
     3361 caggtcgagc cacctgcaca cagtgactcc ctggaccccc agaccaacag ccaacagcag
     3421 accacacagt acatcatcac caccaccacc aacgggaacg gaagcagcga agtgcatatc
     3481 accaaaccat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     3541 atatcttgtg gaaaggacga gtgctcgacc aacgcagatg ccta
//
